New insights into the regulation of cathepsin K gene expression by osteoprotegerin ligand
New insights into the regulation of cathepsin K gene expression by osteoprotegerin ligand
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DOI：
10.1006/bbrc.2001.5127
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发表时间：
2001-07-13
影响因子：
3.1
通讯作者：
Troen, BR
中科院分区：
文献类型：
--
作者：
Corisdeo, S;Gyda, M;Troen, BR
组织蛋白酶K在骨吸收中起关键作用。我们提供了第一个证据，骨保护素配体（OPGL），一个关键的促吸收细胞因子，急性刺激组织蛋白酶K在破骨细胞中的表达。采用原位RT-PCR和实时定量RT-PCR检测组织蛋白酶K基因的表达。OPGL提高大鼠新生长骨成熟破骨细胞组织蛋白酶K mRNA水平。OPGL联合巨噬细胞集落刺激因子（M-CSF）也能刺激骨髓培养的单核细胞和多核破骨细胞中组织蛋白酶K基因的表达。实时定量RT-FCR显示骨髓培养物中组织蛋白酶K mRNA水平较高，与破骨细胞发生的程度相当。因此，我们认为OPGL至少在一定程度上通过诱导组织蛋白酶K基因表达来促进骨吸收。(C) 2001学术出版社。
Cathepsin K plays a key role in bone resorption. We provide the first evidence that osteoprotegerin ligand (OPGL), a critical pro-resorptive cytokine, acutely stimulates the expression of cathepsin K in osteoclasts. We used in situ RT-PCR and real time quantitative RT-PCR to analyze cathepsin K gene expression. OPGL enhanced cathepsin K mRNA levels in mature osteoclasts isolated from rat neonatal long bones. OPGL together with macrophage colony-stimulating factor (M-CSF) also stimulated cathepsin K gene expression in monocytic cells and multinucleate osteoclasts in bone marrow cultures. Real time quantitative RT-FCR demonstrated high levels of cathepsin K mRNA in bone marrow cultures, paralleling the degree off osteoclastogenesis. We therefore suggest that OPGL enhances bone resorption, at least in part, by inducing cathepsin K gene expression. (C) 2001 Academic Press.