ACTIVATORS OF PROTEIN KINASE-C DOWN-REGULATE AND PHOSPHORYLATE THE T3/T-CELL ANTIGEN RECEPTOR COMPLEX OF HUMAN LYMPHOCYTES-T
ACTIVATORS OF PROTEIN KINASE-C DOWN-REGULATE AND PHOSPHORYLATE THE T3/T-CELL ANTIGEN RECEPTOR COMPLEX OF HUMAN LYMPHOCYTES-T
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DOI：
10.1073/pnas.82.23.8158
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
CRUMPTON, MJ
中科院分区：
文献类型：
--
作者：
CANTRELL, DA;DAVIES, AA;CRUMPTON, MJ
通过间接免疫荧光法判断，佛波醇12，13-二丁酸酯和1-油酰基-2-乙酰基甘油诱导人T淋巴母细胞上T3抗原的表面表达以及HPB-ALL细胞上T3和T细胞抗原受体的表面表达快速、浓度依赖性地降低约50%。使用125I标记的抗体的直接结合实验表明，T3表达的减少对应于抗原分子数量的减少，而不是其亲和力的变化。生物化学分析显示佛波醇二丁酸酯诱导T3 Mr 26，000 γ的快速、显著的磷酸化。链以及较小程度的Mr 21，000 δ。链T3 ε无磷酸化链或α链。和β检测T细胞抗原受体的亚基。数据表明蛋白激酶C诱导T3 γ的磷酸化。和δ可能导致T3/T细胞抗原受体复合物下调的链。
As judged by indirect immunofluorescence, phorbol 12,13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct binding experiments using 125I-labeled antibody indicated that the reduction in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 Mr 26,000 .gamma. chain and to a lesser extent of the Mr 21,000 .delta. chain. No phosphorylation of the T3 .epsilon. chain or of the .alpha. and .beta. subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 .gamma. and .delta. chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex.