Ameliorative effect of animal bile preparations on dextran sulfate sodium-induced colitis in mice
Ameliorative effect of animal bile preparations on dextran sulfate sodium-induced colitis in mice
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动物胆汁制剂对右旋糖酐硫酸钠诱导的小鼠结肠炎的改善作用
DOI：
10.1002/tkm2.1094
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Imura Johji
中科院分区：
文献类型：
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作者：
Watanabe Shiro;Fujita Kyosuke;Nishida Takeshi;Imura Johji
目的从熊、牛和猪中提取的动物胆汁制剂由不同类型的胆汁酸组成。鉴于几种类型的胆汁酸激活法尼醇X受体（FXR）或武田G蛋白受体5（TGR 5），从而发挥抗炎作用，我们比较了三种动物胆汁制剂对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的影响。MethodsBear bile（BB），cattle bile（CB），and pig bile（PB）在DSS治疗期间以100 mg/kg/day口服给药7天。结肠炎的症状和炎症反应的组织学和生化标志物在结肠tissues.ResultsBB，CB和PB同样改善DSS治疗小鼠的结肠炎症状。BB和CB而不是PB减弱了DSS处理的小鼠的结肠组织中的炎症反应。BB和CB显著增加了胆汁酸激动FXR和TGR 5的含量，但PB没有增加。结论BB，CB和PB在改善DSS治疗小鼠的结肠炎症状方面没有区别。然而，三种动物胆汁制剂对结肠炎症作用的差异可能是由于抗炎胆汁酸含量的差异。
AimAnimal bile preparations harvested from bears, cattle, and pigs are composed of distinct types of bile acids. Given that several types of bile acid activate the farnesoid X receptor (FXR) or Takeda G‐protein receptor 5 (TGR5) and thereby exert anti‐inflammatory effects, we compared the effects of the three animal bile preparations on dextran sulfate sodium (DSS)‐induced colitis in mice.MethodsBear bile (BB), cattle bile (CB), and pig bile (PB) were orally given at 100 mg/kg/day during DSS treatment for 7 days. The colitis symptoms and the histological and biochemical markers of inflammatory response in the colon tissues were assessed.ResultsBB, CB, and PB similarly ameliorated the colitis symptoms in DSS‐treated mice. BB and CB but not PB attenuated inflammatory response in the colon tissues of DSS‐treated mice. The content of the bile acids agonistic to FXR and TGR5 was significantly increased by BB and CB but not by PB.ConclusionBB, CB, and PB are indistinguishable in ameliorating colitis symptoms in DSS‐treated mice. The differences, however, in the effects of the three animal bile preparations on colon inflammation may be due to the differences in the content of anti‐inflammatory bile acids.