Stimulus‐secretion coupling: the concept and clues from chromaffin and other cells
Stimulus‐secretion coupling: the concept and clues from chromaffin and other cells
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刺激分泌耦合：嗜铬细胞和其他细胞的概念和线索
DOI：
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发表时间：
1968
影响因子：
7.3
通讯作者：
W. Douglas
中科院分区：
文献类型：
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作者：
W. Douglas
本讲座总结了一个领域的知识称为autopharmacology：分泌的一种物质，由内部刺激促使体细胞。这里报道的大部分工作是关于肾上腺嗜铬细胞的。主题包括描述和证明分泌物释放到嗜铬细胞的细胞外环境是嗜铬细胞的细胞内记录手段和乙酰胆碱对膜电位的影响以及将细胞暴露于乙酰胆碱的后果（改变膜化学，增加钠和钙离子的膜渗透性进入和去极化）。还假设了2种可能的儿茶酚胺分泌机制（两者都涉及通过颗粒和细胞粘附膜的直接破裂（即胞吐作用）或通过粘附部位的某些显著增加的渗透性直接从嗜铬颗粒释放到细胞外部）。讲座然后集中在钙和分泌耦合在其他系统，包括外分泌腺，神经元和神经分泌细胞，肥大细胞和多形核白细胞。在关于钙在分泌机制中的作用的结论性讨论中，提到暴露于乙酰胆碱的肾上腺流出物中存在前列腺素，作为分泌机制是化学反应的证据。
This lecture summarizes the knowledge in a field dubbed autopharmacology: the secretion by a body cell of a substance prompted by an internal stimulus. The majority of the work reported on here was with the adrenal chromaffin cell. Topics covered describing and proving the release of secretion into the chromaffin cells extracellular environment are means of intracellular recording from chromaffin cells and the effect of acetylcholine on membrane potential and consequences of exposing the cell to acetylcholine (altered membrane chemistry increased membrane permeability entry of sodium and calcium ions and depolarization). Also postulated are 2 possible mechanisms of catecholamine secretion (both involve release directly from chromaffin granules to the cell exterior through frank rupture of the adherent membranes of granule and cell i.e. exocytosis or through some greatly increased permeability at the adhesion site). The lecture then focuses on calcium and stumulus secretion coupling in other systems including the exocrine glands neurones and neurosecretory cells the mast cell and polymorphonuclear leukocytes. In a concluding discussion on the role of calcium in secretory mechanisms the presence of prostaglandin in effluent from adrenal glands exposed to acetylcholine is mentioned as evidence of the secretory mechanism being a chemical reaction.