Coordination of Glucosinolate Biosynthesis and Turnover Under Different Nutrient Conditions
Coordination of Glucosinolate Biosynthesis and Turnover Under Different Nutrient Conditions
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DOI：
10.3389/fpls.2019.01560
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发表时间：
2019-12-06
影响因子：
5.6
通讯作者：
Burow, Meike
中科院分区：
文献类型：
--
作者：
Jeschke, Verena;Weber, Konrad;Burow, Meike
动态变化的环境条件促进了植物代谢的复杂调节和平衡的资源投资，以发展和防御。紫茎泽兰目的植物组成性地分配碳、氮和硫来合成硫代葡萄糖苷作为它们的主要防御代谢物。先前的研究结果支持一个模型，其中在完整组织中的硫代葡萄糖苷的稳态水平是由生物合成和营业额通过一个尚未表征的营业额途径。为了研究硫代葡萄糖苷在没有组织损伤的情况下的周转，我们在不同的营养条件下定量了外源应用的烯丙基硫代葡萄糖苷和内源硫代葡萄糖苷。我们的数据表明，在幼苗的拟南芥登记哥伦比亚-0，硫代葡萄糖苷的生物合成和营业额协调根据营养供应。而外源性碳源硫苷积累的一般定量影响，硫或氮限制硫苷配置文件中的显着变化，表明这些常量营养素提供不同的监管投入。萝卜糖酸，一种可能由所有硫代葡萄糖苷结构形成的分解产物，似乎不反映植物中的周转率，而是与内源性硫代葡萄糖苷积累增加相关。因此，萝卜芦酸可以代表代谢检查点，其允许产生芥子油苷的植物测量通过生物合成和/或周转途径的通量，从而响应于内部和外部信号动态地调节芥子油苷积累。
Dynamically changing environmental conditions promote a complex regulation of plant metabolism and balanced resource investments to development and defense. Plants of the Brassicales order constitutively allocate carbon, nitrogen, and sulfur to synthesize glucosinolates as their primary defense metabolites. Previous findings support a model in which steady-state levels of glucosinolates in intact tissues are determined by biosynthesis and turnover through a yet uncharacterized turnover pathway. To investigate glucosinolate turnover in the absence of tissue damage, we quantified exogenously applied allyl glucosinolate and endogenous glucosinolates under different nutrient conditions. Our data shows that, in seedlings of Arabidopsis thaliana accession Columbia-0, glucosinolate biosynthesis and turnover are coordinated according to nutrient availability. Whereas exogenous carbon sources had general quantitative effects on glucosinolate accumulation, sulfur or nitrogen limitation resulted in distinct changes in glucosinolate profiles, indicating that these macronutrients provide different regulatory inputs. Raphanusamic acid, a breakdown product that can potentially be formed from all glucosinolate structures appears not to reflect in planta turnover rates, but instead correlates with increased accumulation of endogenous glucosinolates. Thus, raphanusamic acid could represent a metabolic checkpoint that allows glucosinolate-producing plants to measure the flux through the biosynthetic and/or turnover pathways and thereby to dynamically adjust glucosinolate accumulation in response to internal and external signals.