A new efficient expression system for Bacillus and its application to production of recombinant phytase
A new efficient expression system for Bacillus and its application to production of recombinant phytase
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DOI：
10.1023/a:1005694731039
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发表时间：
2000-08-01
影响因子：
2.7
通讯作者：
Miasnikov, A
中科院分区：
文献类型：
--
作者：
Kerovuo, J;von Weymarn, N;Miasnikov, A
建立了一个新的枯草芽孢杆菌表达系统。该系统使用穿梭载体（B.枯草杆菌（subtilis）-大肠杆菌（eutheriticiacoli）），其携带磷酸盐饥饿诱导型启动子（PST），并采用补料分批培养策略。pst启动子被证明是非常强的，并且当存在于多拷贝质粒上时也保持其紧密调控。将该表达系统应用于重组植酸酶的生产，培养终了时植酸酶活力达到28.7 U ml（-1）。
A new expression system was developed for Bacillus subtilis. This system uses a shuttle vector (B. subtilis - Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillus phytase - phytase activity at the end of cultivation reached 28.7 U ml(-1).