EFFECTS OF NATURAL INTERFERON-ALPHA, NATURAL TUMOR-NECROSIS-FACTOR-ALPHA AND THEIR COMBINATION ON HUMAN MESOTHELIOMA XENOGRAFTS IN NUDE-MICE
EFFECTS OF NATURAL INTERFERON-ALPHA, NATURAL TUMOR-NECROSIS-FACTOR-ALPHA AND THEIR COMBINATION ON HUMAN MESOTHELIOMA XENOGRAFTS IN NUDE-MICE
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DOI：
10.1007/bf01789128
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发表时间：
1993-01-01
影响因子：
5.8
通讯作者：
MINOWADA, J
中科院分区：
文献类型：
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作者：
OHNUMA, T;SZRAJER, L;MINOWADA, J
在三种人间皮瘤细胞系裸鼠移植瘤上，检测了单独的人天然干扰素α（nIFN）、单独的人天然肿瘤坏死因子α（nTNF）及其组合（OH-1）的作用。在处理第-12天通过套管针皮下移植肿瘤。腹膜内（i. p.）剂量为2 x 10（7）或2 x 10（8）IU/kg，每日1次，每周5天，持续3周。i.给予nTNF。p.剂量为2 × 10（7）或2 × 10（8）U kg-1 day-1，给药方案与nIFN相同。连续测量肿瘤直径并计算肿瘤体积。通过两种方法评估抗肿瘤作用：比较治疗组和对照组的最终肿瘤体积（T/C），以及中位平均总肿瘤体积的变化。治疗未产生临床可识别的毒性。nIFN对所有三种人间皮瘤细胞系具有强的抑制活性。单独的nTNF仅在使用的高剂量下具有适度的活性。两者的组合产生的活性基本上类似于单独使用nIFN产生的活性。高剂量的nIFN可能作为一种活性药物在间皮瘤患者的治疗中发挥作用。
Effects of human natural interferon alpha (nIFN) alone, human natural tumor necrosis factor alpha (nTNF) alone and their combination (OH-1) were tested on three human mesothelioma lines implanted in nude mice. Tumors were transplanted subcutaneously by trocar on treatment day -12. nIFN was given intraperitoneally (i.p.) at a dose of 2 x 10(7) or 2 x 10(8) IU kg-1 day-1, 5 days a week for 3 weeks. nTNF was given i. p. at a dose of 2 X 10(7) or 2 x 10(8) U kg-1 day-1 in the same schedule as that of nIFN. Tumor diameters were serially measured and tumor volumes were calculated. Antitumor effects were assessed by two methods: comparison of final tumor volumes in treated and control groups (T/C), and changes in median average total tumor volume. The treatment produced no clinically discernible toxicities. nIFN had strong inhibitory activity against all three human mesothelioma lines. nTNF alone had modest activity only at the high dose used. The combination of the two produced activity essentially similar to that produced by nIFN alone. High-dose nIFN may have a role as an active agent in the treatment of patients with mesothelioma.