Inheritance and localisation of resistance to Mycosphaerella graminicola causing septoria tritici blotch and plant height in the wheat (Triticum aestivum L.) genome with DNA markers
Inheritance and localisation of resistance to Mycosphaerella graminicola causing septoria tritici blotch and plant height in the wheat (Triticum aestivum L.) genome with DNA markers
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DOI：
10.1007/s00122-003-1276-2
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发表时间：
2003-08-01
影响因子：
5.4
通讯作者：
Jahoor, A
中科院分区：
文献类型：
--
作者：
Eriksen, L;Borum, F;Jahoor, A
对小麦壳针孢斑病的抗性，禾生球腔菌Mycosphaerella graminicola（Fuckel.）J. Schrot in Cohn.（无性型Septoria triangulae Robbery in Desmaz.）以感病冬小麦品种萨凡纳和抗病品种Senat杂交的双单倍体（DH）群体为材料，研究了小麦抗白粉病的抗性。构建了包括76个SSR位点和244个AFLP位点的遗传连锁图谱。测定了亲本和DH后代对M.在田间试验中，在幼苗阶段在生长室中对禾本科生的分离物混合物进行分离，并且在成株阶段对分离物混合物进行分离。位于或接近Stb 6基因座映射到染色体3A的基因提供幼苗抗IPO 323。两个互补的基因，定位到染色体3A，其中之一是IPO 323抗性基因，需要对丹麦分离株Riso 97 -86的抗性。此外，一些次要位点影响生长室中抗性的表达。在田间试验中检测到4个抗枯斑病的QTL。位于3A和6 B染色体上的两个QTL分别解释了两次试验平均表型方差的18.2%和67.9%。Riso 97 -86在苗期也检测到这两个QTL，而IPO 323只在3A上检测到QTL。另外，在成株期检测到的2个QTL位于2B和7 B染色体上，在苗期未检测到。在2B、3A、3B染色体上和未分配到染色体上的连锁群上检测到4个株高QTL。在3A和未分配的连锁群上的主效QTL在两次试验中一致，并且3A上的QTL似乎与一个抗壳针孢斑点病的QTL连锁。
Resistance to the disease septoria tritici blotch of wheat (Triticum aestivum L.), caused by the fungus Mycosphaerella graminicola (Fuckel.) J. Schrot in Cohn (anamorph Septoria tritici Roberge in Desmaz.) was investigated in a doubled-haploid (DH) population of a cross between the susceptible winter wheat cultivar Savannah and the resistant cultivar Senat. A molecular linkage map of the population was constructed including 76 SSR loci and 244 AFLP loci. Parents and DH progeny were tested for resistance to single isolates of M. graminicola in a growth chamber at the seedling stage, and to an isolate mixture at the adult plant stage, in field trials. A gene located at or near the Stb6 locus mapping to chromosome 3A provided seedling resistance to IPO323. Two complementary genes, mapping to chromosome 3A, one of which was the IPO323 resistance gene, were needed for resistance to the Danish isolate Riso97-86. In addition, a number of minor loci influenced the expression of resistance in the growth chamber. In the field, four QTLs for resistance to septoria tritici blotch were detected. Two QTLs, located on chromosomes 3A and 6B explained 18.2 and 67.9% of the phenotypic variance in the mean over two trials. Both these QTLs were also detected at the seedling stage with isolate Riso97-86, whereas isolate IPO323 only detected the QTL on 3A. Additionally, two QTLs identified in adult plants on chromosomes 2B and 7B were not detected at the seedling stage. Four QTLs were detected for plant height located on chromosomes 2B, 3A, 3B and on a linkage group not assigned to a chromosome. The major QTLs on 3A and on the unassigned linkage group were consistent over two trials, and the QTL on 3A seemed to be linked to a QTL for septoria tritici blotch resistance.