Genome-wide DNA methylation profiling in rheumatoid arthritis identifies disease-associated methylation changes that are distinct to individual T- and B-lymphocyte populations
Genome-wide DNA methylation profiling in rheumatoid arthritis identifies disease-associated methylation changes that are distinct to individual T- and B-lymphocyte populations
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DOI：
10.4161/epi.29718
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发表时间：
2014-09-01
期刊：
影响因子：
3.7
通讯作者：
Farrell, William E.
中科院分区：
文献类型：
--
作者：
Glossop, John R.;Emes, Richard D.;Farrell, William E.
类风湿性关节炎（RA）患者的DNA甲基化组的变化已被描述。在以前的工作中，我们报道了健康个体的T淋巴细胞和B淋巴细胞群体的全基因组甲基化差异。现在，使用HumanMethylation 450 BeadChips询问全基因组DNA甲基化，我们已经确定了12名血清阳性RA女性患者血液来源的T和B淋巴细胞群体中疾病相关的甲基化变化，相对于12名匹配的健康个体。使用NIMBL软件分析阵列数据，并使用亚硫酸氢盐焦磷酸测序来验证阵列候选物。通过分析LINE-1序列确定的全基因组DNA甲基化显示，与T淋巴细胞相比，B淋巴细胞的甲基化程度更高（P
Changes to the DNA methylome have been described in patients with rheumatoid arthritis (RA). In previous work, we reported genome-wide methylation differences in T-lymphocyte and B-lymphocyte populations from healthy individuals. Now, using HumanMethylation450 BeadChips to interrogate genome-wide DNA methylation, we have determined disease-associated methylation changes in blood-derived T-and B-lymphocyte populations from 12 female patients with seropositive established RA, relative to 12 matched healthy individuals. Array data were analyzed using NIMBL software and bisulfite pyrosequencing was used to validate array candidates. Genome-wide DNA methylation, determined by analysis of LINE-1 sequences, revealed higher methylation in B-lymphocytes compared with T-lymphocytes (P