Expression of prostaglandin E2 prostanoid receptor EP2 and interleukin-1β in laryngeal carcinoma - preliminary study.
Expression of prostaglandin E2 prostanoid receptor EP2 and interleukin-1β in laryngeal carcinoma - preliminary study.
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DOI：
10.5114/wo.2015.51417
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Starska K
中科院分区：
文献类型：
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作者：
Mochocki M;Morawski P;Kopta R;Brzezińska-Błaszczyk E;Stasikowska O;Lewy-Trenda I;Student Scientific Circle;Starska K
肿瘤微环境炎症细胞和肿瘤细胞产生的 EP2 蛋白（前列腺素 E2 (PGE2) 受体）的表达可能以自分泌和旁分泌方式促进细胞增殖和生长。涉及这些蛋白质的现象受到白细胞介素 1β (IL-1β) 的调节。许多研究人员指出，EP2 和 IL-1β 在各种类型的肿瘤中存在关联，导致肿瘤进展较快且预后较差。本研究的目的是分析喉癌组织中EP2的表达和外周血单个核细胞上清液中IL-1β的水平，并寻找临床形态学特征之间的关系。本研究对 50 名确诊为鳞状细胞喉癌的患者进行了分析。病理学评估包括根据肿瘤前沿分级标准的pTNM浸润深度。使用免疫组织化学分析对肿瘤组织中EP2的膜染色进行分析。通过酶联免疫吸附测定（ELISA）测定IL-1β的表达。对于更晚期的肿瘤，癌细胞中 EP2 表达增加得到证实（pT3-pT4 与 pT1-pT2，p < 0.0001 和 pN1-3 与 pN0，p = 0.02）。通过更深入地侵入粘膜下层或软骨而确定的具有最高侵袭性的肿瘤的特征是EP2的最高表达（p<0.0001）。在以低分化为特征的喉癌中，肿瘤细胞中 EP2 表达最高（p = 0.009）。 IL-1β 表达与淋巴结转移之间的正相关关系也得到证实 (p = 0.04)。该研究表明 EP2 受体和 IL-1β 对喉癌肿瘤进展的潜在影响。
Expression of EP2 protein, the prostaglandin E2 (PGE2) receptor, produced by tumour microenvironment inflammatory cells as well as tumour cells, may promote cellular proliferation and growth in an autocrine and paracrine fashion. The phenomenon involving these proteins is regulated by interleukin 1β (IL-1β). Many researchers indicate a connection of EP2 and IL-1β in various types of neoplasms with higher tumour progression and poor prognosis. The aim of this study was to analyse the EP2 expression within laryngeal carcinoma tissue and IL-1β levels in peripheral blood mononuclear cell supernatants and to find relationships between clinicomorphological features. A group of 50 patients with verified squamous cell laryngeal carcinoma was analysed in this study. The pathological evaluation included pTNM depth of invasion according to tumour front grading criteria. Immunohistochemical analysis for membranous staining of EP2 in tumour tissues was used. The IL-1β expression was determined by enzyme-linked immunosorbent assay (ELISA). Increased EP2 expression in carcinoma cells was confirmed for more advanced tumours (pT3-pT4 vs. pT1-pT2, p < 0.0001 and pN1-3 vs. pN0, p = 0.02). Tumours with the highest aggressiveness identified by deeper invasion of submucosa or cartilage were characterised by the highest expression of EP2 (p < 0.0001). In laryngeal carcinomas characterised by a lower differentiation the highest EP2 expression in tumour cells was noted (p = 0.009). A positive relationship between IL-1β expression and the presence of lymph node metastases was also confirmed (p = 0.04). The study indicates the potential effect of EP2 receptor and IL-1β on tumour progression in laryngeal carcinoma.