The acquisition of multidimensional NMR spectra within a single scan
The acquisition of multidimensional NMR spectra within a single scan
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DOI：
10.1073/pnas.252644399
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发表时间：
2002-12-10
影响因子：
11.1
通讯作者：
Lupulescu, A
中科院分区：
文献类型：
--
作者：
Frydman, L;Scherf, T;Lupulescu, A
介绍并举例说明了一种能够在一次连续采集中对多维核磁共振结构域进行完整采样的方案。只要分析物的信号足够强，多维核磁共振实验的采集时间就可以缩短几个数量级。这可以对蛋白质折叠、样品上的2D核磁共振实验等瞬时事件进行表征，将高维实验(例如4D核磁共振)的持续时间引入大多数蛋白质在生理条件下的寿命，并促进将光谱2D序列纳入活体成像研究。该协议与现有的多维脉冲序列兼容，并可通过使用常规硬件实现；其性能在此以各种同核2D核磁共振采集为例进行了说明。
A scheme enabling the complete sampling of multidimensional NMR domains within a single continuous acquisition is introduced and exemplified. Provided that an analyte's signal is sufficiently strong, the acquisition time of multidimensional NMR experiments can thus be shortened by orders of magnitude. This could enable the characterization of transient events such as proteins folding, 2D NMR experiments on samples being chromatographed, bring the duration of higher dimensional experiments (e.g., 4D NMR) into the lifetime of most proteins under physiological conditions, and facilitate the incorporation of spectroscopic 2D sequences into in vivo imaging investigations. The protocol is compatible with existing multidimensional pulse sequences and can be implemented by using conventional hardware; its performance is exemplified here with a variety of homonuclear 2D NMR acquisitions.