Changes in sulfate-reducing bacterial populations during the onset of black band disease
Changes in sulfate-reducing bacterial populations during the onset of black band disease
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DOI：
10.1038/ismej.2010.143
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发表时间：
2011-03-01
期刊：
影响因子：
11
通讯作者：
Sato, Yui
中科院分区：
文献类型：
--
作者：
Bourne, David G.;Muirhead, Andrew;Sato, Yui
促进珊瑚黑带病（BBD）发病的因素仍然难以捉摸，尽管复杂微生物垫下的缺氧条件和硫化物的产生与底层珊瑚组织坏死有关。本研究利用实时荧光定量PCR （RT-PCR）和针对异亚硫酸盐还原酶（dsrA）基因的克隆方法，研究了BBD发病过程中硫酸盐还原细菌（SRB）种群的多样性和数量变化。针对16S rRNA基因的定量pcr （qPCR）测定也提供了细菌总数的估计，并允许确定病灶内SRB的相对百分比。三个Montipora sp.珊瑚菌落被鉴定为病变，以前称为蓝藻斑块（CPs）（包括与BBD病变不同的微生物群落），被标记并随着时间的推移跟踪CP发展为BBD。dsra靶向qPCR在CP样本中检测到的基因拷贝数很少（< 65 / ng DNA），尽管在BBD病变中拷贝数增加（> 2500 / ng DNA）。CP样品中的SRB不到细菌种群的1%，但占BBD种群的7.5%。克隆文库也显示，当病变从CP转移到BBD时，优势的dsrA序列发生了变化。本研究结果证实，SRB在BBD发病期间增加，可能增加了微生物垫底部的硫化物浓度，促进了BBD的发病。ISME Journal (2011) 5,559 -564；doi: 10.1038 / ismej.2010.143;2010年9月2日在线发布
Factors that facilitate the onset of black band disease (BBD) of corals remain elusive, though anoxic conditions under the complex microbial mat and production of sulfide are implicated in necrosis of underlying coral tissues. This study investigated the diversity and quantitative shifts of sulfate-reducing bacterial (SRB) populations during the onset of BBD using real-time PCR (RT-PCR) and cloning approaches targeting the dissimilatory (bi) sulfite reductase (dsrA) gene. A quantitative-PCR (qPCR) assay targeting the 16S rRNA gene also provided an estimate of total bacteria, and allowed the relative percentage of SRB within the lesions to be determined. Three Montipora sp. coral colonies identified with lesions previously termed cyanobacterial patches (CPs) (comprising microbial communities unlike those of BBD lesions), were tagged and followed through time as CP developed into BBD. The dsrA-targeted qPCR detected few copies of the gene in the CP samples (< 65 per ng DNA), though copy numbers increased in BBD lesions (> 2500 per ng DNA). SRB in CP samples were less than 1% of the bacterial population, though represented up to 7.5% of the BBD population. Clone libraries also demonstrated a shift in the dominant dsrA sequences as lesions shifted from CP into BBD. Results from this study confirm that SRB increase during the onset of BBD, likely increasing sulfide concentrations at the base of the microbial mat and facilitating the pathogenesis of BBD. The ISME Journal (2011) 5, 559-564; doi: 10.1038/ismej.2010.143; published online 2 September 2010