Confirmation of assignment of the trichloromethyl radical spin adduct detected by spin trapping during 13C-carbon tetrachloride metabolism in vitro and in vivo.
Confirmation of assignment of the trichloromethyl radical spin adduct detected by spin trapping during 13C-carbon tetrachloride metabolism in vitro and in vivo.
复制标题
确认 13C-四氯化碳体外和体内代谢过程中通过自旋捕获检测到的三氯甲基自由基自旋加合物的归属。
DOI：
10.1016/0006-291x(80)90540-9
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发表时间：
1980
影响因子：
3.1
通讯作者：
E. R. Davis
中科院分区：
文献类型：
--
作者：
J. Poyer;P. McCay;Edward K. Lai;E. Janzen;E. R. Davis
含有自由基自旋陷阱、苯基叔丁基硝酮以及NADPH发生系统和[13C]CCl4(90原子%13C)的大鼠肝微粒体孵育系统产生的电子自旋共振谱与三氯甲基-苯基-叔丁基硝酮加合物的电子自旋共振谱一致。在口服[13C]CCl4的大鼠肝脏的脂肪提取物以及经紫外光照射的苯基叔丁基硝酮和[13C]CCl4的溶液中也观察到了同样的光谱。
Rat liver microsomal incubation systems containing the free radical spin trap, phenyl-t-butyl nitrone, as well as an NADPH generating system and [13C]CCl4(90 atom %13C) produce electron spin resonance spectra consistent with that expected for a trichloromethyl-phenyl-t-butyl nitrone adduct. This same spectrum is observed in a lipid extract of the liver from a rat orally administered [13C]CCl4as well as in a solution of phenyl-t-butyl nitrone and [13C]CCl4irradiated with ultraviolet light.