Mitochondrial DNA content varies with pathological characteristics of breast cancer.
Mitochondrial DNA content varies with pathological characteristics of breast cancer.
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DOI：
10.1155/2011/496189
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发表时间：
2011
影响因子：
--
通讯作者：
Wong LJ
中科院分区：
文献类型：
--
作者：
Bai RK;Chang J;Yeh KT;Lou MA;Lu JF;Tan DJ;Liu H;Wong LJ
癌症中线粒体DNA（mtDNA）含量的变化已被报道，其结果存在争议，可能是由于样本量小和病理条件多变。本研究对302例乳腺癌组织和癌旁正常组织中mtDNA含量进行了检测，并与肿瘤的临床病理特征进行了相关性分析。总体而言，肿瘤组织中mtDNA含量明显低于周围正常组织，P < 0.00001。肿瘤组织中mtDNA含量随肿瘤体积增大而降低。然而，当肿瘤非常大（>50 cm 3）时，线粒体DNA含量开始增加。同样，mtDNA含量从0级和I级到II级肿瘤降低，但从II级到III级肿瘤增加。线粒体DNA编码区改变的肿瘤细胞线粒体DNA含量显著高于无改变的肿瘤细胞（P < 0.001）。在D-Loop区有体细胞mtDNA改变的肿瘤中，mtDNA含量显著降低（P < 0.001）。肿瘤组织中mtDNA含量低和高的患者比mtDNA含量中位数的患者死亡风险明显更高。肿瘤组织中的mtDNA含量随肿瘤大小、分级和ER/PR状态而变化; mtDNA含量中位数的显著偏离与不良生存相关。
Changes in mitochondrial DNA (mtDNA) content in cancers have been reported with controversial results, probably due to small sample size and variable pathological conditions. In this study, mtDNA content in 302 breast tumor/surrounding normal tissue pairs were evaluated and correlated with the clinico-pathological characteristics of tumors. Overall, mtDNA content in tumor tissues is significantly lower than that in the surrounding normal tissues, P < 0.00001. MtDNA content in tumor tissues decreased with increasing tumor size. However, when the tumor is very large (>50 cm3), mtDNA content started to increase. Similarly, mtDNA content decreased from grades 0 and I to grade II tumors, but increased from grade II to grade III tumors. Tumors with somatic mtDNA alterations in coding region have significantly higher mtDNA content than tumors without somatic mtDNA alterations (P < 0.001). Tumors with somatic mtDNA alterations in the D-Loop region have significantly lower mtDNA content (P < 0.001). Patients with both low and high mtDNA content in tumor tissue have significantly higher hazard of death than patients with median levels of mtDNA content. mtDNA content in tumor tissues change with tumor size, grade, and ER/PR status; significant deviation from the median level of mtDNA content is associated with poor survival.