Increased oxidative stress and effects on inflammatory cytokine secretion by heated tobacco products aerosol exposure to mice
Increased oxidative stress and effects on inflammatory cytokine secretion by heated tobacco products aerosol exposure to mice
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DOI：
10.1016/j.bbrc.2022.04.042
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发表时间：
2022-04-21
影响因子：
3.1
通讯作者：
Hattori, Kenji
中科院分区：
文献类型：
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作者：
Sawa, Marie;Ushiyama, Akira;Hattori, Kenji
虽然与传统的可燃香烟烟雾相比，加热烟草制品（HTPs）气溶胶中的化学物质含量有所减少，但HTPs与健康影响减少之间的关联仍不清楚。在这项研究中，我们假设暴露于IQOS（一种HTP）会增加氧化应激并影响炎症细胞因子的分泌。首先，评价暴露于IQOS气溶胶的C57 BL/6小鼠，以确定IQOS暴露的不良影响。IQOS暴露可显著降低肺泡巨噬细胞内GSH含量，并呈剂量依赖性，增加肺组织中GSSG的百分比。这些结果表明，IQOS暴露增加氧化应激，GSH被消耗以消除氧化应激。此外，IQOS暴露后，在支气管肺泡灌洗液中观察到泡沫状肺泡巨噬细胞。尽管IQOS暴露后血浆中炎性细胞因子IL-6和GM-CSF的浓度显著增加，但其他细胞因子无显著变化。这些结果表明，短期暴露于IQOS气溶胶可能会增加氧化应激，并诱导炎症细胞因子的分泌。最后，今后应评估IQOS气溶胶暴露的长期影响。(c)2022爱思唯尔公司All rights reserved.
Although the amount of chemicals in heated tobacco products (HTPs) aerosols is reduced compared to conventional combustible cigarette smoke, the association between HTPs and reduced health effects remains unclear. In this study, we hypothesized that exposure to IQOS, an HTP, would increase oxidative stress and affect the secretion of inflammatory cytokines. First, C57BL/6 mice exposed to IQOS aerosols were evaluated to determine the adverse effects of IQOS exposure. IQOS exposure significantly decreased the concentration of GSH in alveolar macrophages in a dose-dependent manner and increased the percentage of GSSG in lung tissues. These results indicate that IQOS exposure increases oxidative stress, and GSH is consumed to remove oxidative stress. In addition, foamy alveolar macrophages were observed in the bronchial alveolar lavage fluid after IQOS exposure. Although the concentration of in-flammatory cytokines, IL-6, and GM-CSF, in the plasma increased significantly after IQOS exposure, there were no significant changes in other cytokines. These results indicate that short-term exposure to IQOS aerosols may increase oxidative stress and induce the secretion of inflammatory cytokines. Lastly, the longer-term effects of IQOS aerosols exposure should be evaluated in the future. (c) 2022 Elsevier Inc. All rights reserved.