DNA detection by surface enhanced resonance Raman scattering (SERRS)
DNA detection by surface enhanced resonance Raman scattering (SERRS)
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DOI：
10.1039/b500248f
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发表时间：
2005-01-01
期刊：
影响因子：
4.2
通讯作者：
Graham, D
中科院分区：
文献类型：
--
作者：
Faulds, K;Smith, WE;Graham, D
这篇教育文章概述了表面增强共振拉曼散射（SERRS）可用于检测DNA的不同方法。介绍了各种不同的SERRS检测策略的使用，这些策略允许获得敏感和选择性检测。通过SERRS检测DNA涉及使用与DNA一起的染料，无论是作为插入物还是通过直接共价连接。这会产生强烈的SERRS信号，表明特定DNA序列的存在。本文还讨论了SERRS检测DNA在不同分子生物学分析中的应用。
This Education article outlines the different ways in which surface enhanced resonance Raman scattering (SERRS) can be used for the detection of DNA. The use of various different SERRS detection strategies that have allowed both sensitive and selective detection to be obtained is covered. Detection of DNA by SERRS involves the use of a dye with the DNA, whether as an intercalator or by direct covalent attachment. This generates strong SERRS signals that indicate the presence of the specific DNA sequence. The SERRS detection of DNA in different molecular biological assays is also discussed.