Streptacidiphilus fuscans sp. nov., a novel actinobacterium isolated from the root of pumpkin ( Cucurbita moschata)
Streptacidiphilus fuscans sp. nov., a novel actinobacterium isolated from the root of pumpkin ( Cucurbita moschata)
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嗜褐链球菌 sp.
DOI：
10.1099/ijsem.0.004824
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Xiangjing Wang
中科院分区：
文献类型：
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作者：
Bing Yu;Chuanyu Han;Junwei Zhao;Yuting Zhang;Qiqi Shan;Yan Wu;Hanxun Ju;Wensheng Xiang;Xiangjing Wang
从采自黑龙江省牡丹江市的南瓜根中分离到一株新的嗜酸性放线菌，命名为NEAU-YB 345 T。根据16 S rRNA基因序列的相似性以及化学分类学和形态学特征，将该菌株归属于嗜黑链霉菌属（Streptacidiphilus），与产黑链霉菌JCM 16224 T（99.2%）、嗜安链霉菌JCM 16223 T（99.1%）和嗜江西链霉菌JCM 12277 T（98.7%）的16 S rRNA基因序列相似性较高。 其细胞壁含有11-二氨基庚二酸作为主要的二氨基酸。从全细胞水解物中检测到的糖为鼠李糖、核糖、葡萄糖和半乳糖。磷脂谱由二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和未鉴别的磷脂组成。甲基萘醌为MK-9（H8）和MK-9（H6）。主要脂肪酸为C16：0、iso-C16：0、iso-C15：0和antiso-C15：0。 利用16 S rRNA基因和全基因组序列的系统发育分析将该菌株置于不同的分支中，但在Streptacidiphilus属内。DNA G+C含量为71.2mol%。根据DNA-DNA亲缘关系和生理生化数据，该分离物可以与其最近的亲戚区分开来。因此，菌株NEAU-YB 345 T代表了嗜酸链球菌属的一个新种，其名称为Streptacidiphilus fuscanssp.十一是建议。模式菌株为NEAU-YB 345 T（=CCTCC AA 2020030 T =JCM 33976 T）。
A novel acidophilic actinobacterium, designated strain NEAU-YB345T, was isolated from a pumpkin root collected from Mudanjiang, Heilongjiang Province, northeast PR China. Based on 16S rRNA gene sequence similarity and chemotaxonomic and morphological properties, the isolate was assigned to the genusStreptacidiphilus, with the high 16S rRNA gene sequence similarities toStreptacidiphilus melanogenesJCM 16224T(99.2 %),Streptacidiphilus anmyonensisJCM 16223T(99.1 %) andStreptacidiphilus jiangxiensisJCM 12277T(98.7 %). Its cell wall containedll-diaminopimelic acid as the major diamino acid. Rhamnose, ribose, glucose and galactose were the detected sugars from the whole-cell hydrolysates. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified phospholipid. The menaquinones were MK-9(H8) and MK-9(H6). Major fatty acids were C16 : 0, iso-C16 : 0, iso-C15 : 0and anteiso-C15 : 0. Phylogenetic analysis using 16S rRNA gene and whole-genome sequences placed the strain in distinct clades but within the genusStreptacidiphilus. The DNA G+C content was 71.2 mol%. Based on DNA–DNA relatedness and physiological and biochemical data, the isolate could be distinguished from its closest relatives. Therefore, strain NEAU-YB345Trepresents a novel species of the genusStreptacidiphilus, for which the nameStreptacidiphilus fuscanssp. nov. is proposed. The type strain is NEAU-YB345T(=CCTCC AA 2020030T=JCM 33976T).