Induction of oocyte maturation and changes in the biochemical composition, physiology and molecular biology of oocytes during maturation and hydration in the orange-spotted grouper (Epinephelus coioides)
Induction of oocyte maturation and changes in the biochemical composition, physiology and molecular biology of oocytes during maturation and hydration in the orange-spotted grouper (Epinephelus coioides)
复制标题
橙斑石斑鱼（Epinephelus coioides）卵母细胞成熟的诱导以及成熟和水合过程中卵母细胞生化成分、生理学和分子生物学的变化
DOI：
10.1016/j.aquaculture.2020.735115
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发表时间：
2020-05-30
期刊：
影响因子：
4.5
通讯作者：
Li, Shuisheng
中科院分区：
文献类型：
--
作者：
Tang, Lin;Xiao, Xinxun;Li, Shuisheng
在水产养殖中，卵母细胞成熟、排卵和产卵对成功繁殖和种子生产至关重要。大多数海洋硬骨鱼可以通过肌肉注射人绒毛膜促性腺激素（HCG）来诱导产卵。在本研究中，采用卵巢内注射的方法来解决黄斑鱼对HCG无反应的问题。单侧注射卵巢的卵母细胞在35小时内成功成熟并排卵。建立了体外诱导hcg诱导卵母细胞成熟的方法。HCG或成熟诱导激素（MIHs）诱导直径在490 ~ 560 μ m之间的卵母细胞成熟，并对卵母细胞成熟和水化过程中的生化、生理和分子变化进行了表征。在卵母细胞成熟过程中，除半胱氨酸外，其他游离氨基酸的浓度均呈上升趋势，Na+则呈下降趋势，K+呈上升趋势。编码组织蛋白酶B、D、L以及Na+、K+- atp酶亚基的基因在水化过程中表达上调。利用组织蛋白酶和Na+， K+ - atp酶的特异性抑制剂进一步证实了它们在体内和体外卵母细胞水化中的重要作用。这些结果为海洋硬骨鱼诱导卵母细胞成熟和评价卵母细胞质量提供了有价值的数据。
In aquaculture, oocyte maturation, ovulation and spawning are important for successful breeding and seed production. Most marine teleost fish can be induced to spawn regularly using intramuscular injections of human chorionic gonadotropin (HCG). In this study, an intra-ovarian injection method was developed in orange-spotted grouper to deal with the broodstocks unresponsive to HCG. Successful oocyte maturation and ovulation in the unilateral injected ovary was induced within 35 h of treatment. An in vitro method was then established to induce HCG-primed oocyte maturation. HCG or maturation-inducing hormones (MIHs) induced the maturation of oocytes with a diameter between 490 and 560 mu m. Biochemical, physiological and molecular changes during oocyte maturation and hydration were also characterized. The concentration of free amino acids except the cysteine increased during oocyte maturation, whereas Na+ decreased steadily and K+ increased. The expression of genes encoding cathepsin B, D, L, and the subunits of Na+ , K+-ATPase were upregulated during hydration. Specific inhibitors for cathepsin and Na+ , K+ -ATPase were used to further confirm their crucial roles in oocyte hydration both in vivo and in vitro. These results provide valuable data for inducing oocyte maturation and evaluating oocyte quality in marine teleost fish.