Traditional Chinese drug ShuXueTong facilitates angiogenesis during wound healing following traumatic brain injury
Traditional Chinese drug ShuXueTong facilitates angiogenesis during wound healing following traumatic brain injury
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DOI：
10.1016/j.jep.2008.02.033
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发表时间：
2008-05-22
影响因子：
5.4
通讯作者：
Bie, Xiaodong
中科院分区：
文献类型：
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作者：
Jin, Xiaoying;Shen, Gang;Bie, Xiaodong
民族药理相关性：疏血通注射液是一种传统中药，用于治疗包括亚急性脑损伤在内的血瘀证患者。然而，疏血通对创伤性脑损伤的治疗作用机制尚不清楚。研究目的：我们假设疏血通可能通过促进血管生成来促进脑创伤的愈合。材料和方法：采用微血管计数、Western blotting、免疫细胞化学、四甲基偶氮唑蓝比色法等方法，研究疏血通对脑创伤后血管生成的影响，进而探讨其对血管内皮生长因子/血管内皮生长因子受体-2通路的影响。结果：疏血通促进了创伤大鼠脑内血管生成，改善了神经功能。疏血通诱导后，皮损中血管内皮生长因子的表达增加。体外实验显示，疏血通作用1d后，血管内皮细胞VEGFR-2和SRF的表达明显增强，并且通过血管内皮生长因子/血管内皮生长因子-2途径促进内皮细胞的增殖。结论：疏血通治疗创伤性脑损伤的作用机制至少部分是通过促进损伤区血管生成来实现的。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
Ethnopharmacological relevance: ShuXueTong injection is a traditional Chinese drug designed to treat the patients of "blood stasis and stagnation (yu xue yu zhi)", including subacute brain trauma. However, the mechanism of the therapeutic effect of ShuXueTong on traumatic brain injury is unknown yet.Aim of the study: We hypothesized that ShuXueTong may promote the brain wound healing by facilitating angiogenesis. Thus this study was designed to explore this hypothesis.Materials and methods: By means of microvessel count, Western blotting, immunocytochemistry, methyl thiazolyl tetrazolium assay and etc., the effect of ShuXueTong on the angiogenesis of brain wound was studied and then its influence on the VEGF/VEGFR-2 pathway were explored.Results: ShuXueTong facilitates angiogenesis in the brain wound and improves the neurological function of the traumatized rats. VEGF expression in the lesion was elevated due to ShuXueTong induction. The in vitro experiment revealed VEGFR-2 and SRF expression in the endothelial cells were enhanced when exposed to ShuXueTong for merely 1 d. Moreover, ShuXueTong promoted the endothelial cell proliferation via the VEGF/VEGFR-2 pathway.Conclusions: The mechanism of the therapeutic effect of ShuXueTong on traumatic brain injury lies at least partly in the enhanced angiogenesis in the lesion. (C) 2008 Elsevier Ireland Ltd. All rights reserved.