Bioethanol production: an integrated process of low substrate loading hydrolysis-high sugars liquid fermentation and solid state fermentation of enzymatic hydrolysis residue.
Bioethanol production: an integrated process of low substrate loading hydrolysis-high sugars liquid fermentation and solid state fermentation of enzymatic hydrolysis residue.
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DOI：
10.1016/j.biortech.2012.07.118
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发表时间：
2012-11
影响因子：
11.4
通讯作者：
Qiulu Chu;Xin Li;B. Ma;Yong Xu;Ouyang Jia;Junjun Zhu;Shiyuan Yu;Q. Yong
中科院分区：
文献类型：
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作者：
Qiulu Chu;Xin Li;B. Ma;Yong Xu;Ouyang Jia;Junjun Zhu;Shiyuan Yu;Q. Yong
研究了一种酶解-发酵一体化工艺，以实现乙醇的高产。低底物负荷下的酶解、高糖浓度的液体发酵和酶解残渣的固态发酵相结合有利于蒸汽爆破预处理玉米秸秆转化为乙醇。结果表明，低底物负荷水解可获得较高的酶解产率;酿酒酵母液体发酵200 g/L葡萄糖可获得较高的乙醇浓度，可显著降低后续乙醇蒸馏的成本。将酶解残渣与固态发酵相结合，可提高乙醇产量和纤维素转化率。固态发酵试验结果表明，固态发酵过程伴随着同步糖化发酵过程。
An integrated process of enzymatic hydrolysis and fermentation was investigated for high ethanol production. The combination of enzymatic hydrolysis at low substrate loading, liquid fermentation of high sugars concentration and solid state fermentation of enzymatic hydrolysis residue was beneficial for conversion of steam explosion pretreated corn stover to ethanol. The results suggested that low substrate loading hydrolysis caused a high enzymatic hydrolysis yield; the liquid fermentation of about 200g/L glucose by Saccharomyces cerevisiae provided a high ethanol concentration which could significantly decrease cost of the subsequent ethanol distillation. A solid state fermentation of enzymatic hydrolysis residue was combined, which was available to enhance ethanol production and cellulose-to-ethanol conversion. The results of solid state fermentation demonstrated that the solid state fermentation process accompanied by simultaneous saccharification and fermentation.