Chromosomal assignment and deletion mapping of barley EST markers
Chromosomal assignment and deletion mapping of barley EST markers
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DOI：
10.1266/ggs.80.357
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发表时间：
2005-10-01
影响因子：
1.1
通讯作者：
Endo, TR
中科院分区：
文献类型：
--
作者：
Nasuda, S;Kikkawa, Y;Endo, TR
从大约10000个大麦EST标记中筛选出1421个EST标记，它们在小麦(Triticum aestivum cv.)中国春)和大麦(Hordeum vocgare cv.)贝茨)。我们利用一组小麦-大麦染色体附加系进行了PCR分析，将它们分配到了7条大麦染色体上(1H到7H)。成功地将701个(49.3%)EST标记定位到大麦染色体上：75~1H、127~2H、119~3H、94~4H、108~5H、81~6H和97~7H。利用一组中国春的Betze大麦端体附加系，我们成功地确定了每条大麦染色体上至少90%的EST标记的染色体臂(S或L)的位置。我们利用分配给7HS(49)或7HL(41)的90个EST标记和19个携带7H结构变化的小麦品系进行了初步定位。远端EST标记较近端多。
From about 10000 PCR-based EST markers of barley we chose 1421 EST markers that were demonstrated to be amplified differently by PCR between wheat (Triticum aestivum cv. Chinese Spring) and barley (Hordeum vulgare cv. Betzes). We assigned them to the seven barley chromosomes (1H to 7H) by PCR analysis using a set of wheat-barley chromosome addition lines. We successfully assigned 701 (49.3%) EST markers to the barley chromosomes: 75 to 1H, 127 to 2H, 119 to 3H, 94 to 4H, 108 to 5H, 81 to 6H and 97 to 7H. By using a set of Betzes barley telosomic addition lines of Chinese Spring, we could successfully determine the chromosome-arm (S or L) location of at least 90% of the EST markers assigned to each barley chromosome. We conducted a trial mapping using 90 EST markers assigned to 7HS (49) or 7HL (41) and 19 wheat lines carrying 7H structural changes. More EST markers were found in the distal region than in the proximal region.