Novel Insights into Tat Pathway in Xanthomonas oryzae pv. oryzae Stress Adaption and Virulence: Identification and Characterization of Tat-Dependent Translocation Proteins
Novel Insights into Tat Pathway in Xanthomonas oryzae pv. oryzae Stress Adaption and Virulence: Identification and Characterization of Tat-Dependent Translocation Proteins
复制标题
对米黄单胞菌 (Xanthomonas oryzae pv.) 中 Tat 途径的新见解。
DOI：
10.1094/phyto-02-17-0053-r
复制
发表时间：
2017
期刊：
影响因子：
3.2
通讯作者：
Liu Fengquan
中科院分区：
文献类型：
--
作者：
Wu Guichun;Su Panpan;Wang Bo;Zhang Yuqiang;Qian Guoliang;Liu Fengquan
黄oryzaepv。Oryzae是一种经济上重要的细菌，在世界范围内的水稻生产中引起一种严重的疾病，称为细菌性叶枯病。HowX。oryzaepv。稻瘟病毒感染水稻并引起症状仍不完全清楚。我们早期的工作表明，双精氨酸易位（Tat）途径在x中起着至关重要的作用。oryzaepv。水稻的适应性和毒力，但潜在的机制尚不清楚。在本研究中，我们以菌株pxo99aa为工作模型，通过比较蛋白质组学和生物信息学分析鉴定了15个潜在的tat依赖性易位蛋白（TDTP）。结合系统诱变、表型表征和基因表达，我们发现多个TDTP在x中起关键作用。oryzaepv。水稻适应性或毒力。其中，4个TDTP （PXO_02203、PXO_03477、PXO_02523和PXO_02951）参与毒力作用，3个TDTP （PXO_02203、PXO_03477和PXO_02523）参与植物定殖，1个TDTP （PXO_02671）参与叶片表面附着，4个TDTP （PXO_02523、PXO_02951、PXO_03132和PXO_03841）参与多重胁迫耐受，2个TDTP （PXO_02523和PXO_02671）参与完整的群体运动。这些发现表明，多个TDTP可能对参与x的Tat通路有不同的贡献。oryzaepv。水稻的适应性、生理学和致病性。
Xanthomonas oryzaepv.oryzae, an economically important bacterium, causes a serious disease in rice production worldwide called bacterial leaf blight. HowX. oryzaepv.oryzaeinfects rice and causes symptoms remains incompletely understood. Our earlier works demonstrated that the twin-arginine translocation (Tat) pathway plays an vital role inX. oryzaepv.oryzaefitness and virulence but the underlying mechanism is unknown. In this study, we used strain PXO99Aas a working model, and identified 15 potential Tat-dependent translocation proteins (TDTP) by using comparative proteomics and bioinformatics analyses. Combining systematic mutagenesis, phenotypic characterization, and gene expression, we found that multiple TDTP play key roles inX. oryzaepv.oryzaeadaption or virulence. In particular, four TDTP (PXO_02203, PXO_03477, PXO_02523, and PXO_02951) were involved in virulence, three TDTP (PXO_02203, PXO_03477, and PXO_02523) contributed to colonization in planta, one TDTP (PXO_02671) had a key role in attachment to leaf surface, four TDTP (PXO_02523, PXO_02951, PXO_03132, and PXO_03841) were involved in tolerance to multiple stresses, and two TDTP (PXO_02523 and PXO_02671) were required for full swarming motility. These findings suggest that multiple TDTP may have differential contributions to involvement of the Tat pathway inX. oryzaepv.oryzaeadaption, physiology, and pathogenicity.