VINBLASTINE-INDUCED BLOCKAGE OF ORTHOGRADE AND RETROGRADE AXONAL-TRANSPORT OF PROTEIN IN RETINAL GANGLION-CELLS
VINBLASTINE-INDUCED BLOCKAGE OF ORTHOGRADE AND RETROGRADE AXONAL-TRANSPORT OF PROTEIN IN RETINAL GANGLION-CELLS
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DOI：
10.1016/0014-4886(74)90119-8
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发表时间：
1974-01-01
影响因子：
5.3
通讯作者：
LUND, RD
中科院分区：
文献类型：
--
作者：
BUNT, AH;LUND, RD
成年白化病大鼠右眼玻璃体内注射0.1-30 μg硫酸长春碱，左眼同样注射生理盐水。24小时后，双眼注射H3-脯氨酸，两个上级丘注射辣根过氧化物酶。24小时后，处死动物，处理脑和视网膜，用于放射自显影和过氧化物酶的显示。0.5 μg或更高剂量的长春碱可阻断视神经轴突内放射性标记蛋白质的快速顺行转运，而不会导致视网膜神经节细胞中蛋白质合成的显著减少。需要更高剂量的长春碱（10 μg或更高）来阻断过氧化物酶从上级丘到神经节细胞胞体的逆行轴突运输。这表明长春碱敏感的机制，蛋白质的顺行和逆行轴突运输，其中可能涉及微管或轴浆内的游离微管蛋白，因为长春碱在低浓度下特异性结合微管蛋白。
The right eyes of adult, albino rats were injected intravitreally with 0.1–30 μg of vinblastine sulfate, and the left eyes were similarly injected with saline. Twenty-four hours later, both eyes were injected with H3-proline and both superior colliculi with horseradish peroxidase. After 24 hr, the animals were killed and brains and retinae processed both for radioautography and the demonstration of peroxidase. Doses of 0.5 μg or more of vinblastine produced blockage of rapid orthograde transport of radioactively labeled protein within optic nerve axons without causing a significant decrease in protein synthesis in ganglion cells of the retina. A higher dose of vinblastine (10 μg or more) was required to block retrograde axonal transport of peroxidase from the superior colliculus to the ganglion cell somata. This suggests vinblastine-sensitive mechanisms for both orthograde and retrograde axonal transport of protein which may involve microtubules or free tubulin within the axoplasm, since vinblastine in low concentration binds specifically to tubulin.