Border cell polarity and collective migration require the spliceosome component Cactin.
Border cell polarity and collective migration require the spliceosome component Cactin.
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DOI：
10.1083/jcb.202202146
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发表时间：
2022-07-04
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影响因子：
--
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中科院分区：
文献类型：
--
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果蝇边缘细胞是一种已建立的集体细胞迁移的体内模型。Miao等人表明，剪接体C复合物组分Cactin对于协调集体迁移细胞之间的顶基极性是必不可少的，并且迁移细胞比静止的上皮细胞对扰动的极性更敏感。边界细胞是集体细胞迁移的体内模型。在这里，我们确定的基因cactin作为必不可少的边界细胞簇组织，分层和迁移。在Cactin耗尽的细胞中，顶端蛋白aPKC和Crumbs（Crb）变得异常集中，并且整体簇极性丧失。顶端拴系过量的aPKC足以引起分层缺陷，并且重新定位顶端aPKC部分地挽救分层。从酵母到人类，Cactin是保守的，并参与了多种过程。在边缘细胞中，需要Cactin进化上保守的剪接体功能。全转录组分析揭示了肌动蛋白耗竭细胞中同种型表达的改变。在两个受影响的基因，Sec23和Sec24CD，交通Crb顶端细胞表面的突变，部分拯救边缘细胞簇的组织和迁移。Rab5或Rab11的过表达促进Crb和aPKC再循环，类似地挽救。因此，一个通用的剪接因子是特别需要的集群极性和迁移的协调，和迁移的边界细胞特别敏感的剪接和细胞极性中断。
Drosophila border cells are an established in vivo model for collective cell migration. Miao et al. show that a spliceosome C complex component, Cactin, is essential to coordinate apicobasal polarity among collectively migrating cells and that migrating cells are more sensitive to perturbed polarity than stationary epithelial cells. Border cells are an in vivo model for collective cell migration. Here, we identify the gene cactin as essential for border cell cluster organization, delamination, and migration. In Cactin-depleted cells, the apical proteins aPKC and Crumbs (Crb) become abnormally concentrated, and overall cluster polarity is lost. Apically tethering excess aPKC is sufficient to cause delamination defects, and relocalizing apical aPKC partially rescues delamination. Cactin is conserved from yeast to humans and has been implicated in diverse processes. In border cells, Cactin’s evolutionarily conserved spliceosome function is required. Whole transcriptome analysis revealed alterations in isoform expression in Cactin-depleted cells. Mutations in two affected genes, Sec23 and Sec24CD, which traffic Crb to the apical cell surface, partially rescue border cell cluster organization and migration. Overexpression of Rab5 or Rab11, which promote Crb and aPKC recycling, similarly rescues. Thus, a general splicing factor is specifically required for coordination of cluster polarity and migration, and migrating border cells are particularly sensitive to splicing and cell polarity disruptions.
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影响因子：
1.2
作者：
Bach, Erika A.;Ekas, Laura A.;Baeg, Gyeong-Hun
通讯作者：
Baeg, Gyeong-Hun
DOI：
10.1083/jcb.201007095
发表时间：
2011-01-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hatan M;Shinder V;Israeli D;Schnorrer F;Volk T
通讯作者：
Volk T
影响因子：
4.6
作者：
Borensztejn, Antoine;Boissoneau, Elisabeth;Pret, Anne-Marie
通讯作者：
Pret, Anne-Marie
DOI：
10.1073/pnas.1524448113
发表时间：
2016-05-03
影响因子：
11.1
作者：
Au, Sam H.;Storey, Brian D.;Toner, Mehmet
通讯作者：
Toner, Mehmet
影响因子：
64.5
作者：
Geisbrecht, ER;Montell, DJ
通讯作者：
Montell, DJ