Skin Inflammation and Testicular Function: Dermatitis Causes Male Infertility via Skin-Derived Cytokines.
Skin Inflammation and Testicular Function: Dermatitis Causes Male Infertility via Skin-Derived Cytokines.
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DOI：
10.3390/biomedicines8090293
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发表时间：
2020-08-20
期刊：
影响因子：
4.7
通讯作者：
Yamanaka K
中科院分区：
文献类型：
--
作者：
Umaoka A;Takeuchi H;Mizutani K;Seo N;Matsushima Y;Habe K;Hagimori K;Yamaguchi Y;Ikeda T;Yamanaka K
包括皮肤病在内的医学合并症与男性不育有关。男性不育最常见的原因是睾丸不能产生精子;然而，持续性皮炎对睾丸功能的影响迄今尚未阐明。我们使用自发性皮炎小鼠模型研究了皮肤炎症和精子生成受损之间的关系。我们检查了皮炎小鼠及其野生型同窝小鼠的繁殖记录。通过直接显微镜观察和流式细胞术分析精子计数、运动力和存活力。此外，对睾丸和附睾进行组织学检查。最后，在另一种皮炎小鼠模型和腹腔内给予炎性细胞因子的野生型小鼠中评价精子活力。与野生型同窝小鼠相比，患有皮炎的小鼠中出生的儿童数量较低。体重和睾丸大小呈年龄依赖性下降。在皮肤病组中，精子计数和运动率明显降低，并观察到精子活力降低。组织学检查显示支持细胞脱落，精子发生减少。附睾间质纤维化严重，可能影响附睾内缺陷精子的成熟。此外，这种现象通过应用半抗原的皮炎小鼠模型和腹腔内施用炎性细胞因子来再现。一旦皮肤发炎，就会产生和释放炎性细胞因子，这可能会影响睾丸和精子功能。男性不育症与人类严重皮炎的关系尚需进一步研究。
The medical comorbidities including skin diseases are associated with male infertility. The most common cause of male infertility is the inability of testes to produce sperm; however, the influence of persistent dermatitis on testicular function has not been elucidated so far. We investigated the relationship between skin inflammation and impaired sperm production using a spontaneous dermatitis mouse model. We examined the breeding records of dermatitis mice and their wild-type littermates. Sperm count, motility, and viability were analyzed by direct microscopic observation and flow cytometry. In addition, testis and epididymis were histologically examined. Finally, sperm viability was evaluated in another dermatitis mouse model and in wild-type mice in which inflammatory cytokines were intraperitoneally administered. Compared to wild-type littermate mice, the number of children born was lower in mice with dermatitis. The body weight and testis size were decreased age-dependently. In the skin disease group, the sperm count and movement ratio were clearly decreased, and reduced sperm viability was observed. Histological examination revealed the detachment of Sertoli cells and reduced spermatogenesis. The fibrosis of epididymal stroma was severe, and it might affect defective sperm maturation in the epididymis. In addition, this phenomena was reproduced by a hapten applied dermatitis mouse model and the intraperitoneal administration of inflammatory cytokines. Once the skin is inflamed, inflammatory cytokines are produced and released, which may affect testicular and sperm function. Additional studies are needed to determine the relationship between male infertility and severe dermatitis in human.
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影响因子：
5.6
作者：
Mizutani, Kento;Shirakami, Eri;Yamanaka, Keiichi
通讯作者：
Yamanaka, Keiichi
影响因子：
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通讯作者：
van der Poel, S.
影响因子：
6.7
作者：
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通讯作者：
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