Improved computer program data for the resolution and fractionation of macromolecules by isokinetic sucrose density gradient sedimentation.
Improved computer program data for the resolution and fractionation of macromolecules by isokinetic sucrose density gradient sedimentation.
复制标题
通过等速蔗糖密度梯度沉降改进了大分子分辨率和分级的计算机程序数据。
DOI：
10.1016/0003-2697(74)90343-1
复制
发表时间：
1974
影响因子：
2.9
通讯作者：
K. S. McCarty
中科院分区：
文献类型：
--
作者：
K. McCarty;R. Vollmer;K. S. McCarty
计算机计算的等速梯度为已知密度（l-3）的大分子和颗粒的区域离心提供了上级均匀分辨率。该技术特别有用，因为它能够提供沉降系数，而无需繁琐的常规计算，并且不需要使用内标（2）。此外，该技术可用于预测两种已知密度的大分子或颗粒的分辨率所需的离心时间。通过使用计算机程序，还可以计算沉降系数和在任何预选梯度上的区域沉降（4）。
Computer calculated isokinetic gradients provide superior uniform resolution for zone centrifugation of macromolecules and particles of known density (l-3). The technique is particularly useful in that it has the capacity to provide sedimentation coefficients without cumbersome routine computations and does not necessitate the use of internal standards (2). In addition, the technique can be utilized to predict the required centrifugation time for the resolution of two macromolecules or particles of known density. With the use of computer programs it is also possible to calculate sedimentation coefficients and zone sedimentation on any preselected gradient (4).