Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations.
Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations.
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DOI：
10.1002/cne.23995
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发表时间：
2016-10-01
期刊：
影响因子：
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通讯作者：
Dacey DM
中科院分区：
文献类型：
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作者：
Liao HW;Ren X;Peterson BB;Marshak DW;Yau KW;Gamlin PD;Dacey DM
这项研究的长期目标是了解表达黑视素的视网膜神经节细胞，也称为OPN 4，如何有助于人类和其他灵长类动物的视力。在这里，我们报告的结果，解剖学研究，使用我们的多克隆抗体特异性地对人类黑视蛋白，确认和扩展以前的描述黑视蛋白细胞在灵长类动物。在猕猴和人类视网膜中，两个不同的群体的黑视素细胞被确定的基础上树突状分层的内部或外部的内丛状层（IPL）。树突状领域的大小和细胞密度的偏心率的变化得到证实，树突棘，黑视素细胞的一个新的功能，进行了描述。棘是DB 6弥漫性双极细胞轴突终末向内分层型黑视素细胞输入的部位。外部分层黑视蛋白类型通过稀疏的外部轴突轴杆接收来自DB 6双极细胞的输入。外分层黑视蛋白细胞也接受来自多巴胺能无长突细胞轴突末梢的输入。在外层分层的黑视素细胞，带状突触的双极细胞和常规突触的无长突细胞在电子显微镜免疫标记实验中被确定。在外侧膝状体核（LGN）中注射示踪剂后，对内部和外部分层的黑视素细胞类型进行了逆行标记。此外，开发了利用黑视素细胞的固有荧光靶向黑视素细胞用于细胞内注射的方法。该技术被用于证明黑视素细胞与无长突细胞的示踪偶联，并将适用于未来的电生理实验。J. Comp.神经元524：2845-2872，2016。© 2016作者比较神经病学杂志出版的威利期刊，公司.
The long‐term goal of this research is to understand how retinal ganglion cells that express the photopigment melanopsin, also known as OPN4, contribute to vision in humans and other primates. Here we report the results of anatomical studies using our polyclonal antibody specifically against human melanopsin that confirm and extend previous descriptions of melanopsin cells in primates. In macaque and human retina, two distinct populations of melanopsin cells were identified based on dendritic stratification in either the inner or the outer portion of the inner plexiform layer (IPL). Variation in dendritic field size and cell density with eccentricity was confirmed, and dendritic spines, a new feature of melanopsin cells, were described. The spines were the sites of input from DB6 diffuse bipolar cell axon terminals to the inner stratifying type of melanopsin cells. The outer stratifying melanopsin type received inputs from DB6 bipolar cells via a sparse outer axonal arbor. Outer stratifying melanopsin cells also received inputs from axon terminals of dopaminergic amacrine cells. On the outer stratifying melanopsin cells, ribbon synapses from bipolar cells and conventional synapses from amacrine cells were identified in electron microscopic immunolabeling experiments. Both inner and outer stratifying melanopsin cell types were retrogradely labeled following tracer injection in the lateral geniculate nucleus (LGN). In addition, a method for targeting melanopsin cells for intracellular injection using their intrinsic fluorescence was developed. This technique was used to demonstrate that melanopsin cells were tracer coupled to amacrine cells and would be applicable to electrophysiological experiments in the future. J. Comp. Neurol. 524:2845–2872, 2016. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.