Far infrared radiation induces changes in gut microbiota and activates GPCRs in mice
Far infrared radiation induces changes in gut microbiota and activates GPCRs in mice
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DOI：
10.1016/j.jare.2019.12.003
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发表时间：
2020-03-01
影响因子：
10.7
通讯作者：
Hsiao, W. L. Wendy
中科院分区：
文献类型：
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作者：
Khan, Imran;Pathan, Sabrina;Hsiao, W. L. Wendy
远红外辐射（FIR）已被广泛用于治疗慢性疾病和症状;然而，其潜在机制仍不清楚。由于肠道微生物群（GM）显著影响宿主的生理，使GM成为FIR治疗评价的潜在靶标。将C57 BL/6 J小鼠腹部暴露于5次2 min FIR暴露，在一天内每次暴露间隔2 h。在FIR/对照处理后第1天和第25天收集粪便样品，并使用ERIC-PCR和16 S扩增子测序评价提取的粪便DNA。使用qRT-PCR分析宿主的G蛋白偶联受体（GPCR）。FIR引起GM组成的立即变化。与对照组相比，在经FIR辐照的小鼠中观察到脱铁菌门（由几种病原体组成）的丰度迅速且显著（p < 0.05）降低。首先，FIR暴露诱导有益的属，如Alistipes，Baniesiella和Prevotella。短链脂肪酸（SCFAs）生产者占主导地位的FIR照射小鼠的肠道。此外，FIR刺激SCFAs敏感受体GPCR 41，43和109在肠上皮屏障中的表达。这些研究结果提供了第一手证据，其中FIR辐射的有益影响可能部分通过调制GM。(C)2019作者Elsevier B. V.代表开罗大学出版。
Far infrared radiation (FIR) has been widely used to treat chronic diseases and symptoms; however, the underlying mechanism remains unclear. As gut microbiota (GM) markedly impact the host's physiology, making GM a potential target for the therapeutic evaluation of FIR. C57BL/6J mice were exposed to five times of 2 min-FIR exposure on the abdomen, with a two-hour interval of each exposure within one day. Fecal samples were collected on day one and day 25 after the FIR/control treatment, and the extracted fecal DNAs were evaluated using ERIC-PCR and 16S amplicon sequencing. Host's G-protein coupled receptors (GPCR) were analyzed using qRT-PCR. FIR induced immediate changes in the GM composition. A prompt and significant (p < 0.05) reduction in the abundance of phylum Deferribacteres (comprised of several pathogens) was observed in the FIR-irradiated mice compared to the control group. Contrarily, FIR exposure induced beneficial genera such as Alistipes, Baniesiella, and Prevotella. The gut of FIR-irradiated mice was predominated by short-chain fatty acids (SCFAs) producers. Also, FIR stimulated the expression of SCFAs-sensing receptors, GPCR 41, 43, and 109 in the gut epithelial barrier. These findings provide the first-hand evidence in which the beneficial effects of FIR radiation might be partially through the modulation of GM. (C) 2019 The Authors. Published by Elsevier B.V. on behalf of Cairo University.