The High Osmolarity Glycerol (HOG) Pathway Functions in Osmosensing, Trap Morphogenesis and Conidiation of the Nematode-Trapping Fungus Arthrobotrys oligospora.
The High Osmolarity Glycerol (HOG) Pathway Functions in Osmosensing, Trap Morphogenesis and Conidiation of the Nematode-Trapping Fungus Arthrobotrys oligospora.
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DOI：
10.3390/jof6040191
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发表时间：
2020-09-27
期刊：
影响因子：
--
通讯作者：
Hsueh YP
中科院分区：
文献类型：
--
作者：
Kuo CY;Chen SA;Hsueh YP
Hog1是一种丝裂原活化蛋白激酶（MAPK），已在多种真菌物种中发现，它调节各种细胞过程，如渗透适应、营养感知和发病机制。然而，Hog1在捕获线虫的真菌中所起的作用以前并不清楚。在这里，我们鉴定了捕获线虫的真菌Arthrobotrys oligospora中酿酒酵母Hog1和膜粘蛋白Msb2的同源物。我们在少孢单胞菌中产生了HOG1和MSB2基因缺失突变体，并对其在渗透传感、生长和陷阱形态发生中的作用进行了表征。我们发现hog1和msb2突变体对高渗透压非常敏感。捕食分析进一步表明，hog1和msb2的缺失导致陷阱形成和捕食效率的降低。此外，HOG1在A. oligospora的分生过程中是必需的，证明了它在这一发育途径中的关键作用。综上所述，本研究表明，保守的Hog1和Msb2调控了捕获线虫真菌A. oligospora的生理、生长和发育。
Hog1, a mitogen-activated protein kinase (MAPK), has been identified in diverse fungal species, and it regulates various cellular processes, such as osmoadaptation, nutrient-sensing, and pathogenesis. However, the roles that Hog1 plays in nematode-trapping fungi were previously unclear. Here, we characterized orthologs of Saccharomyces cerevisiae Hog1 and membrane mucin Msb2 in the nematode-trapping fungus Arthrobotrys oligospora. We generated gene deletion mutants of HOG1 and MSB2 in A. oligospora, and characterized their roles in osmosensing, growth, and trap morphogenesis. We found that both hog1 and msb2 mutants were highly sensitive to high osmolarity. Predation analyses further revealed that hog1 and msb2 deletion caused a reduction in trap formation and predation efficiency. Furthermore, HOG1 is required for conidiation in A. oligospora, demonstrating its critical role in this developmental pathway. In summary, this study demonstrated that the conserved Hog1 and Msb2 govern physiology, growth and development in the nematode-trapping fungus A. oligospora.
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