The effect of exposure to irrigant solutions on apical dentin biofilms in vitro
The effect of exposure to irrigant solutions on apical dentin biofilms in vitro
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DOI：
10.1016/j.joen.2005.07.002
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发表时间：
2006-05-01
影响因子：
4.2
通讯作者：
Britto, LR
中科院分区：
文献类型：
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作者：
Clegg, MS;Vertucci, FJ;Britto, LR
本研究评估了不同浓度的次氯酸钠 (NaOCl)、2% 洗必泰 (CHX)（Vista Dental Products，拉辛，威斯康星州）和 BioPure MTAD（Dentsply Endodontics-Tulsa Dental，塔尔萨，俄克拉荷马州）的有效性。收集了 10 名诊断为慢性根尖周炎的患者的根管内内容物。将样品培养在根尖的半切片上以产生多种微生物生物膜。将每个生物膜分别浸入 6% NaOCl、3% NaOCl、1% NaOCl、2% CHX、1% NaOCl，然后浸入 BioPure MTAD 和无菌磷酸盐缓冲溶液 (PBS)。 SEM分析显示6％NaOCl和3％NaOCl能够破坏和去除生物膜； 1% NaOCl 和 1% NaCCl，然后使用 MTAD 能够破坏生物膜，但不能消除细菌； 2% CHX 不能破坏生物膜。暴露于 6% WOO、2% CHX 或 1% NaOCl 以及 BioPure MTAD 的样本无法培养出活细菌。这些结果表明，6% NaOCl 是唯一能够使细菌无法存活并物理去除生物膜的冲洗剂。
This study assessed the effectiveness of different concentrations of sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) (Vista Dental Products, Racine, WI), and BioPure MTAD (Dentsply Endodontics-Tulsa Dental, Tulsa, OK). Intracanal contents were collected from 10 patients diagnosed with chronic apical periodontitis. The samples were cultured on hemisections of root apices to generate a polymicrobial biofilm. Each biofilm was separately immersed in 6% NaOCl 3% NaOCl, 1% NaOCl;, 2% CHX, 1% NaOCl followed by BioPure MTAD, and sterile phosphate buffered solution (PBS). SEM analysis showed 6% NaOCl and 3% NaOCl were capable of disrupting and removing the biofilm; 1% NaOCl and 1% NaCCl followed by MTAD were capable of disrupting the biofilm, but not eliminating bacteria; 2% CHX was not capable of disrupting the biofilm. Viable bacteria could not be cultured from specimens exposed to 6% WOO, 2% CHX, or 1% NaOCl followed by BioPure MTAD. These results indicate that 6% NaOCl was the only irrigant capable of both rendering bacteria nonviable and physically removing the biofilm.