UPSTREAM ACTIVATION SITES OF THE CYC1 GENE OF SACCHAROMYCES-CEREVISIAE ARE ACTIVE WHEN INVERTED BUT NOT WHEN PLACED DOWNSTREAM OF THE TATA BOX
UPSTREAM ACTIVATION SITES OF THE CYC1 GENE OF SACCHAROMYCES-CEREVISIAE ARE ACTIVE WHEN INVERTED BUT NOT WHEN PLACED DOWNSTREAM OF THE TATA BOX
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DOI：
10.1073/pnas.81.24.7860
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
HOAR, E
中科院分区：
文献类型：
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作者：
GUARENTE, L;HOAR, E
研究了酵母CYC 1基因上游激活位点（UAS）在倒置或位于TATA盒下游时发挥功能的能力。将带有UAS的130个碱基对的DNA片段倒位，使这些位点的活性几乎完全保持不变。相比之下，将位点定位在TATA盒的下游或CYC 1-核糖体蛋白51-lacZ三杂交体基因的内含子中几乎完全废除了它们的活性。在后一种构建体中，UAS和TATA盒之间的分离大致相当于完整CYC 1启动子区中元件之间的分离。UAS显示不中断该构建体中的转录或剪接，因为位于TATA盒上游的GAL 10 UAS引起三杂交体基因的半乳糖诱导型表达。UAS无法在内含子中发挥作用，部分原因是内含子和TATA盒之间的序列阻止了激活信号。然而，内含子中位点的不活动的一个很大组成部分似乎是它们的下游位置。这一结果进行了讨论，在酵母上游激活的可能机制。
The ability of the upstream activation sites (UASs) of the yeast CYC1 gene to function when inverted or when positioned downstream of the TATA box is investigated. Inversion of a 130 base-pair DNA fragment bearing the UASs leaves the activity of the sites almost completely intact. In contrast, positioning the sites downstream of the TATA box or in the intron of a CYC1-ribosomal protein 51-lacZ tribrid gene almost totally abolishes their activity. In the latter construct, the separation between the UASs and TATA box is roughly equivalent to that between the elements in the intact CYC1 promoter region. The UASs are shown not to interrupt transcription or splicing in this construct since a GAL10 UAS positioned upstream of the TATA box gives rise to galactose-inducible expression of the tribrid gene. The inability of the UASs to function in the intron is partly due to sequences between the intron and the TATA box that block the activation signal. However, a large component of the inactivity of the sites in the intron appears to be their downstream location. This result is discussed in light of possible mechanisms of upstream activation in yeast.