DNA-binding proteins of the Whirly family in Arabidopsis thaliana are targeted to the organelles
DNA-binding proteins of the Whirly family in Arabidopsis thaliana are targeted to the organelles
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DOI：
10.1016/j.febslet.2005.05.059
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发表时间：
2005-07-04
期刊：
影响因子：
3.5
通讯作者：
Krupinska, K
中科院分区：
文献类型：
--
作者：
Krause, K;Kilbienski, I;Krupinska, K
拟南芥含有3个与马铃薯p24高度同源的基因，p24被描述为核转录激活因子Whirly家族的成员。基于计算机的分析显示，所有拟南芥Whirly （Why）蛋白都含有针对质体或线粒体的靶向序列。这些序列的功能被证明是通过体外导入实验分离细胞器。原生质体和洋葱表皮细胞中瞬时表达的GFP融合蛋白证实了这些蛋白分别位于质体或线粒体中。在包括马铃薯p24在内的高等植物物种中，具有细胞器靶向序列的Why蛋白似乎是保守的。(c) 2005年欧洲生化学会联合会。Elsevier B.V.版权所有。
Arabidopsis thaliana contains three genes with high homology to potato p24 which was described as a member of the Whirly family of nuclear transcriptional activators. Computer-based analysis revealed that all Arabidopsis Whirly (Why) proteins contain targeting sequences for either plastids or mitochondria. The functionality of these sequences was demonstrated by in vitro import assays into isolated organelles. Transient expression of GFP fusion proteins in protoplasts and onion epidermal cells confirmed the localisation of these proteins in plastids or mitochondria, respectively. The possession of organellar targeting sequences seems to be conserved among Why proteins of higher plant species, including potato p24. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.