Effect of relaxin on mammary growth in the hypophysectomized rat.
Effect of relaxin on mammary growth in the hypophysectomized rat.
复制标题
松弛素对垂体切除大鼠乳腺生长的影响。
DOI：
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复制
发表时间：
1982
影响因子：
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通讯作者：
R. R. Anderson
中科院分区：
文献类型：
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作者：
L. Wright;R. R. Anderson
卵巢肽松弛素长期以来被认为可以改变妊娠动物产道区域的结构，为分娩做准备（Hall，1960; Hisaw，1926; Hisaw et al，5 1944; Steinetz et al 1960）。由于松弛素在许多怀孕哺乳动物的血清中大量存在，妊娠期间乳腺的发育至少部分地受到该激素的调节似乎是合理的。通过整体观察技术，已经完成了大量描述垂体切除大鼠乳腺生长所需激素的定性研究（Cowie和Folley，1947; Hamolsky和Sparrow，1945; Kahn，Baker和Zannotti，1965; Mixner，刘易斯和Turner，1940）。在过去，该技术适于半定量，最多使用一些任意的数值标度来为每个腺体分配发育程度的等级（Kahn，Baker和Zanotti，1965; Meites，1965; Wrenn等人，1966年）。化学DNA测定是一种可接受的定量方法，尽管在测量间质和实质组织时存在轻微并发症，并且无法获得生长的定性图像。因此，可以得出结论，松弛素对乳腺生长和分化的影响的最佳方法是将先前确定的DNA测量与整体安装观察相结合，并开发更好的定量方法，以进行更有效的统计分析。
The ovarian peptide, relaxin, has long been known to modify the structures in the area of the birth canal of the pregnant animal in preparation for parturition (Hall, 1960; Hisaw, 1926; Hisaw et al,5 1944; Steinetz et al 1960). Since relaxin is found in abundance in the blood serum of many pregnant mammals, it seems plausible that the development of the mammary gland during gestation might be regulated in part at least by the presence of the hormone. Much work in delineating qualitative hormone requirements for mammary growth in hypophysectomized rats has been accomplished through the technique of whole mount observations (Cowie and Folley, 1947; Hamolsky and Sparrow, 1945; Kahn, Baker and Zannotti, 1965; Mixner, Lewis and Turner, 1940). In the past this technique was adapted to semiquantification by at best using some arbitrary numerical scale to assign each gland a grade for the extent of development (Kahn, Baker and Zanotti, 1965; Meites, 1965; Wrenn et al., 1966). Chemical DNA determination is an acceptable method for quantification in spite of the minor complication in measuring stromal as well as parenchymal tissue, and a qualitative picture of growth is not obtained. Therefore, it was concluded that the best approach to investigating the effect of relaxin on growth and differentiation of the mammary gland was to combine previously determined DNA measurements with whole mount observations, and to develop a better method of quantification for a more valid statistical analysis.