Biological Evaluation of the Pollution of Rivers Flowing into Tokyo Bay with the 7-Ethoxycoumarin O-deethylase (ECOD) Activity Induced by River Sediment Extracts in HepG2 Cells
Biological Evaluation of the Pollution of Rivers Flowing into Tokyo Bay with the 7-Ethoxycoumarin O-deethylase (ECOD) Activity Induced by River Sediment Extracts in HepG2 Cells
复制标题
利用河流沉积物提取物在 HepG2 细胞中诱导的 7-乙氧基香豆素 O-脱乙基酶 (ECOD) 活性对流入东京湾的河流污染进行生物学评价
DOI：
10.1248/jhs.47.118
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发表时间：
2001
影响因子：
--
通讯作者：
Y. Inouye
中科院分区：
文献类型：
--
作者：
S. Nito;Natsumi Todoroki;M. Misumi;T. Nakahama;Y. Inouye
使用河流沉积物提取物，在基于HepG 2细胞中芳烃受体（AhR）依赖性诱导7-乙氧基香豆素O-脱乙基酶（ECOD）活性的测定系统中比较研究了流入东京湾的河流的污染程度。河流沉积物的采样点如下：鹤见川的Namamugi，多摩川的Rokugo，阿拉川的Hirai，Senjyu和Funado，江户川的Baraki和花见川的Makuhari（图1）。位于京滨工业区中部的鹤见河（Namamugi）被认为污染最严重，其次是Ara河（Hirai）。在江户河Baraki收集的河流沉积物污染最轻。在Namamugi和Hirai采样的高度污染的河流沉积物的提取物表现出倒U形的剂量-反应曲线，多环芳烃（PAHs）可能是主要负责，在一个无毒浓度范围内。通过查阅中马等人的数据，在Namamugi收集的河流沉积物的污染粗略估计为2 - 20 μg（以PAHs/g沉积物计），而平井的河流沉积物的污染为0.2 - 2 μg（以PAHs/g计）。
The extent of pollution in the rivers flowing into Tokyo Bay was studied comparatively using river sediment extracts in an assay system based on the arylhydrocarbon receptor (AhR)-dependent induction of 7-ethoxycoumarin O-deethylase (ECOD) activity in HepG2 cells. The sampling points of river sediment were as follows: Namamugi on the Tsurumi River, Rokugo on the Tama River, Hirai, Senjyu and Funado on the Ara River, Baraki on the Edo River, and Makuhari on the Hanami River (Fig. 1). The Tsurumi River (Namamugi) located in the middle of Keihin Industrial District was considered most polluted, followed by the Ara River (Hirai). The river sediment collected at Baraki on the Edo River was least polluted. The extracts of highly polluted river sediments sampled at Namamugi and Hirai exhibited a reverse U-shaped dose-response curve, for which polycyclic aromatic hydrocarbons (PAHs) might be mainly responsible, in a range of non-toxic concentrations. The pollution of river sediment collected at Namamugi was roughly estimated to be 2 to 20 μg as PAHs/g sediment by consulting the data of Nakama et al. and 0.2 to 2 μg as PAHs/g was assigned to the river sediment of Hirai.