Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments.
Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments.
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通过免疫细胞动员的水凝胶微球对巨噬细胞进行重编程用于骨关节炎治疗
DOI：
10.1016/j.bioactmat.2023.09.010
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发表时间：
2024-02
影响因子：
18.9
通讯作者：
中科院分区：
文献类型：
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作者：
Regulating macrophage activation precisely is crucial in treating chronic inflammation in osteoarthritis (OA). However, the stable pro-inflammatory state and deep distribution of macrophages in vivo pose a great challenge to treatment. In this study, inspired by the innate immune, immune cell mobilized hydrogel microspheres were constructed by microfluidic methods and load chemokines, macrophage antibodies and engineered cell membrane vesicles (sEVs) via covalent and non-covalent junctions. The immune cell mobilized hydrogel microspheres, based on a mixture of streptavidin grafted hyaluronic acid methacrylate (HAMA-SA) and Chondroitin sulfate methacrylate (ChSMA) microspheres (HCM), can recruit, capture and reprogram proinflammatory macrophages in the joint cavity to improve the joint inflammatory microenvironment. In vitro experiments demonstrated that immune cell mobilized hydrogel microspheres had excellent macrophage recruitment, capture, and reprogramming abilities. Pro-inflammatory macrophages can be transformed into anti-inflammatory macrophages with an efficiency of 88.5 %. Animal experiments also revealed significant reduction in synovial inflammation and cartilage matrix degradation of OA. Therefore, the immune cell mobilized hydrogel microspheres may be an effective treatment of OA inflammation for the future. Inspired by the innate immune, hydrogel microspheres were constructed for mobilizing immune cell, which show excellent macrophage recruitment, capture, and reprogramming abilities. Pro-inflammatory macrophages can be transformed into anti-inflammatory macrophages with an efficiency of 88.5 %. Animal experiments also revealed significant reduction in synovial inflammation and cartilage matrix degradation of osteoarthritis via regulating mitochondrial function. Inflammatory cell recruitment offers a new approach to intraarticular drug delivery. The immune cell mobilized hydrogel microspheres provide macrophage recruitment, capture, and reprogramming abilities. Engineered cell membrane vesicles (sEVs) enhance macrophage reprogramming by repairing mitochondrial dysfunction.
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影响因子：
13.6
作者：
Lei Y;Wang Y;Shen J;Cai Z;Zhao C;Chen H;Luo X;Hu N;Cui W;Huang W
通讯作者：
Huang W
影响因子：
5.8
作者：
Cheng, Zhonghua;Zhu, Yeman
通讯作者：
Zhu, Yeman
肿瘤通过细胞外囊泡劫持了巨噬细胞和菌群。
DOI：
10.1002/exp.20210144
发表时间：
2022-02
期刊：
Exploration (Beijing, China)
影响因子：
--
作者：
Jiang, Jipeng;Mei, Jie;Ma, Yongfu;Jiang, Shasha;Zhang, Jian;Yi, Shaoqiong;Feng, Changjiang;Liu, Yang;Liu, Ying
通讯作者：
Liu, Ying
影响因子：
64.8
作者：
Culemann, Stephan;Grueneboom, Anika;Kroenke, Gerhard
通讯作者：
Kroenke, Gerhard
影响因子：
19
作者：
Lei, Yiting;Wang, Yuping;Huang, Wei
通讯作者：
Huang, Wei