Effect of azelastine on the intracellular Ca2+ mobilization in guinea pig peritoneal macrophages.
Effect of azelastine on the intracellular Ca2+ mobilization in guinea pig peritoneal macrophages.
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氮卓斯汀对豚鼠腹腔巨噬细胞细胞内 Ca2+ 动员的影响。
DOI：
10.1016/0014-2999(88)90451-7
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发表时间：
1988
影响因子：
5
通讯作者：
C. Yamato
中科院分区：
文献类型：
--
作者：
T. Nakamura;Y. Nishizawa;T. Sato;C. Yamato
氮卓斯汀是一种口服有效的抗过敏剂，已被证明可以抑制组胺和白三烯的释放。这表明氮卓斯汀可能会改变细胞内 Ca2+ 的动员。我们使用荧光 Ca2+ 指示剂 fura2 检查了氮卓斯汀对由血小板活化因子 (PAF-acether) 或 N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸 (FMLP) 诱导的豚鼠腹腔巨噬细胞中细胞内游离钙浓度 ([Ca2+])i) 变化的影响。在存在 2 mM EGTA 的情况下，添加 PAF-乙醚后 20 秒内，PAF-乙醚将 [Ca2+]i 从 89 ± 4 提高到 243 ± 26 nM (n = 15)。这表明PAF-乙醚对巨噬细胞的刺激诱导了Ca2+的细胞内动员，并且氮卓斯汀预处理以剂量依赖性方式减少了PAF-乙醚诱导的[Ca2+]i增加(IC50=16μM)。 Azelastine 还抑制 FMLP 诱导的 [Ca2+]i 增加。此外，PAF-乙醚和FMLP均引起巨噬细胞释放前列腺素E2，氮卓斯汀预处理可剂量依赖性地减少PGE2释放（IC50= 10μM）。这些结果表明氮卓斯汀抑制PAF-乙醚或FMLP诱导的细胞内储存位点Ca2+的释放，并且这种作用可能导致细胞中PGE2释放的减少。
Azelastine, an orally effective anti-allergic agent, has been demonstrated to inhibit the release of histamine and leukotrienes. This suggests that azelastine might alter the mobilization of intracellular Ca2+. We have examined the effect of azelastine on the change in intracellular free calcium concentration ([Ca2+])i) in guinea pig peritoneal macrophages induced by platelet activating factor (PAF-acether) or N-formylmethionyl-leucyl-phenylalanine (FMLP) using a fluorescent Ca2+indicator, fura2. PAF-acether raised [Ca2+]ifrom 89 ± 4 to 243 ± 26 nM (n = 15) within 20 s after addition of PAF-acether in the presence of 2 mM EGTA. This indicates that the stimulation of macrophages by PAF-acether induced intracellular mobilization of Ca2+, and pretreatment with azelastine reduced the PAF-acether-induced increase in [Ca2+]iin a dose-dependent manner (IC50= 16μM). Azelastine also inhibited the FMLP-induced increase in [Ca2+]i. Furthermore, PAF-acether and FMLP both caused the release of prostaglandin E2from macrophages, and pretreatment with azelastine reduced the PGE2release dose dependently (IC50= 10μM). These results suggest that azelastine inhibits the release of Ca2+from intracellular storage sites induced by PAF-acether or FMLP, and that this effect possibly causes reduction in the release of PGE2from the cells.
IgE 免疫复合物诱导大鼠肺泡巨噬细胞立即和延长释放白三烯 C4 (LTC4)。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Rankin,JA;Hitchcock,M;Merrill,WW;Huang,SS;Brashler,JR;Bach,MK;Askenase,PW
通讯作者：
Askenase,PW
与 IgG gamma 2b/gamma 1 Fc 受体-配体相互作用相关的细胞内游离钙的增加：在吞噬作用中的作用。
DOI：
10.1073/pnas.81.17.5430
发表时间：
1984
影响因子：
11.1
作者：
Young,JD;Ko,SS;Cohn,ZA
通讯作者：
Cohn,ZA