Low dynamic state of viral competition in a chronic avian hepadnavirus infection
Low dynamic state of viral competition in a chronic avian hepadnavirus infection
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DOI：
10.1128/jvi.74.11.5257-5265.2000
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发表时间：
2000-06-01
影响因子：
5.4
通讯作者：
Summers, J
中科院分区：
文献类型：
--
作者：
Zhang, YY;Summers, J
对11只雏鸭感染鸭乙肝病毒的动态进行了调查。通过接种野生型病毒和具有部分复制缺陷的突变病毒的混合物，在新孵化的雏鸭中建立了慢性感染。不出所料，在感染传播期间，野生型病毒在病毒种群中迅速丰富。此后的浓缩与肝脏的正常生长相关，平均突变型与野生型的比率在肝脏质量稳定后至少2个月保持稳定。使用实验确定的突变型和野生型病毒的生长速度，我们估计在感染传播后，两种病毒株之间的竞争受到感染生长中的肝脏中的新肝细胞所需的复制量的限制。结果表明，在慢性感染的肝脏中，选择具有复制率优势的变异是低效的，这些变异的出现将取决于诱导肝细胞周转，如在慢性肝炎期间发生的那样。
The dynamic state of infection of 11 ducks with the duck hepatitis B virus was investigated. Chronic infections were established in newly hatched ducklings by inoculation with a mixture of wild-type virus and a mutant virus with a partial replication defect. As expected, the wild-type virus was rapidly enriched in the virus population during the spread of infection. Enrichment thereafter was correlated with normal growth of the liver, with the average mutant-to-wild-type ratio stabilizing for at least 2 months beyond the time at which the liver mass stabilized. Using experimentally determined growth rates for the mutant and wild-type viruses, we estimated that after the spread of infection, competition between the two virus strains was limited by the amount of replication required to infect new hepatocytes in the growing livers. The results suggest that, in a chronically infected liver, the selection of variants with a replication rate advantage is inefficient and that the emergence of such variants would depend on induced liver cell turnover, such as that occurring during chronic hepatitis.