A Molecular Blueprint of Gene Expression in Hippocampal Subregions CA1, CA3, and DG is Conserved in the Brain of the Common Marmoset
A Molecular Blueprint of Gene Expression in Hippocampal Subregions CA1, CA3, and DG is Conserved in the Brain of the Common Marmoset
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DOI：
10.1002/hipo.20555
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发表时间：
2009-01-01
期刊：
影响因子：
3.5
通讯作者：
de Kloet, E. R.
中科院分区：
文献类型：
--
作者：
Datson, N. A.;Morsink, M. C.;de Kloet, E. R.
最近对啮齿动物的研究表明，海马亚区CA1、CA3和DG之间的基因表达谱存在显著差异。海马体内分子组成的这些差异很可能是海马体各亚区之间在形态、生理和对侮辱的脆弱性方面的差异的基础，并因此是海马体基本分子结构的一部分。这项研究的目的是大规模地调查这些次区域基因表达的差异是否在一种非人类灵长类动物--普通绒猴的海马体中保守。这项研究是非常及时的，考虑到最近发展的第一个绒猴特异性DNA微阵列，专门包含针对来自绒猴海马体的转录本的序列。通过激光显微切割分离海马亚区CA1、CA3和DG，提取、扩增RNA，并与包含1,500多个在成年恒河猴海马区表达的转录本的绒猴特异微阵列(EUMAMA)杂交。观察到DG区和两个锥体亚区之间的表达差异很大。此外，在单个基因和差异表达程度方面，特定于区域的基因表达模式与啮齿类动物的大脑显示出显著的保守性。据我们所知，这是第一次研究海马区基因在非人灵长类动物中的大规模表达。获得的表达谱不仅为每个海马亚区1500多个转录本的表达提供了新的数据，而且对不同亚区在非人类灵长类大脑中学习和记忆中的作用感兴趣的神经科学家也有潜在的兴趣。(C)2009年Wiley-Liss，Inc.
Recent studies in rodents have shown that there are significant differences in gene expression profiles between the hippocampal subregions CA1, CA3, and DG. These differences in molecular make-up within the hippocampus most likely underlie the differences in morphology, physiology, and vulnerability to insults that exist between the subregions of the hippocampus and are as such part of the basic molecular architecture of the hippocampus. The aim of this study was to investigate at large scale whether these subregional differences in gene expression are conserved in the hippocampus of a nonhuman primate, the common marmoset. This study is very timely, given the recent development of the first marmoset-specific DNA microarray, exclusively containing sequences targeting transcripts derived from the marmoset hippocampus. Hippocampal subregions CA1, CA3, and DG were isolated by laser microdissection and RNA was isolated, amplifed, and hybridized to the marmoset-specific microarray (EUMAMA) containing more than 1,500 transcripts expressed in the adult marmoset hippocampus. Large differences in expression were observed in particular between the DG region and both pyramidal subregions. Moreover, the subregion-specific patterns of gene expression showed a remarkable conservation with the rodent brain both in terms of individual genes and degree of differential expression. To our knowledge, this is the first study investigating large scale hippocampal gene expression in a nonhuman primate. The obtained expression profiles not only provide novel data on the expression of more than 1,500 transcripts per hippocampal subregion but also are of potential interest to neuroscientists interested in the role of the different subregions in learning and memory in the nonhuman primate brain. (C) 2009 Wiley-Liss, Inc.