Dietary rescue of altered metabolism gene reveals unexpected Drosophila mating cues
Dietary rescue of altered metabolism gene reveals unexpected Drosophila mating cues
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DOI：
10.1194/jlr.m064683
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发表时间：
2016-03-01
影响因子：
6.5
通讯作者：
Ferveur, Jean-Francois
中科院分区：
文献类型：
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作者：
Bousquet, Franois;Chauvel, Isabelle;Ferveur, Jean-Francois
为了发育和繁殖，动物需要长链 MUFA 和 PUFA。虽然一些不饱和脂肪酸（UFAs）可以由生物体合成，但其他的则必须由饮食提供。 desat1 基因参与果蝇 UFA 代谢，对于幼虫发育和成虫性信息素通讯都是必需的。我们首先表征了幼虫组织中 desat1 的表达。然后，我们发现 desat1 表达在整个发育过程中被抑制的幼虫在用标准食物饲养时会在幼虫阶段死亡。相比之下，添加到幼虫饮食中的纯 MUFA 或 PUFA（而非饱和 FA）可挽救动物至成年，其中使用油酸 (C18:1) 可获得最佳效果。产前富含 UFA 的饮食对雄性和雌性的交配行为和生育能力的影响非常不同。成虫饮食也强烈影响繁殖的几个方面：与用标准成虫饮食饲养的果蝇相比，用富含 C18:1 的饮食饲养的果蝇表现出更高的交配性能。因此，幼虫和成虫 desat1 表达都控制性别特异性交配信号。类似的营养遗传学方法可能适用于其他代谢突变体，以揭示被严重发育缺陷掩盖的隐秘效应。
To develop and reproduce, animals need longchain MUFAs and PUFAs. Although some unsaturated FAs (UFAs) can be synthesized by the organism, others must be provided by the diet. The gene, desat1, involved in Drosophila melanogaster UFA metabolism, is necessary for both larval development and for adult sex pheromone communication. We first characterized desat1 expression in larval tissues. Then, we found that larvae in which desat1 expression was knocked down throughout development died during the larval stages when raised on standard food. By contrast pure MUFAs or PUFAs, but not saturated FAs, added to the larval diet rescued animals to adulthood with the best effect being obtained with oleic acid (C18:1). Male and female mating behavior and fertility were affected very differently by preimaginal UFA-rich diet. Adult diet also strongly influenced several aspects of reproduction:flies raised on a C18:1-rich diet showed increased mating performance compared with flies raised on standard adult diet. Therefore, both larval and adult desat1 expression control sex-specific mating signals. A similar nutrigenetics approach may be useful in other metabolic mutants to uncover cryptic effects otherwise masked by severe developmental defects.