Cloning and characterization of the 5'UTR of the rat anti-apoptotic Bcl-w gene.
Cloning and characterization of the 5'UTR of the rat anti-apoptotic Bcl-w gene.
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DOI：
10.1016/j.bbrc.2009.09.049
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发表时间：
2009-11-27
影响因子：
3.1
通讯作者：
Chiaramello A
中科院分区：
文献类型：
--
作者：
Uittenbogaard M;Baxter KK;Chiaramello A
The anti-apoptotic Bcl-w regulator, which is expressed in the developing and mature brain, not only promotes neuronal survival, but also neuronal differentiation. However, its transcriptional regulation remains to be elucidated due to a lack of knowledge of the Bcl-w promoter. Here, we report the mapping and characterization of the rat Bcl-w promoter, which is highly conserved between the human, mouse, and rat species. Using a series of 5’ and 3’ deletions, we mapped the TATA-less minimal Bcl-w promoter and showed that it is under a combinatorial regulation with the neurogenic bHLH transcription factor NeuroD6 mediating its activation, validating our previous finding of increased expression of the Bcl-w protein in stably transfected PC12-NeuroD6 cells. Upon stress, NeuroD6 promotes colocalization of Bcl-w with mitochondria and endoplasmic reticulum. Finally, we provide the first evidence of Bcl-w localization in the growth cones of differentiating neuronal cells, suggestive of a potential synaptic neuroprotective role.
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影响因子：
2.5
作者：
Popratiloff, A;Glaume, C;Peusner, KD
通讯作者：
Peusner, KD
影响因子：
5.3
作者：
Yao, MZ;Nguyen, TVV;Pike, CJ
通讯作者：
Pike, CJ
影响因子：
5.3
作者：
Schwab, MH;Bartholomae, A;Nave, KA
通讯作者：
Nave, KA
影响因子：
2.7
作者：
Olson, JM;Asakura, A;Tapscott, SJ
通讯作者：
Tapscott, SJ
影响因子：
4.5
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Liu QA;Shio H
通讯作者：
Shio H