Antigen-presenting cell function during Plasmodium yoelii infection
Antigen-presenting cell function during Plasmodium yoelii infection
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DOI：
10.1128/iai.70.6.2941-2949.2002
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发表时间：
2002-06-01
影响因子：
3.1
通讯作者：
Avery, AC
中科院分区：
文献类型：
--
作者：
Luyendyk, J;Olivas, OR;Avery, AC
抗原呈递细胞（APC）在协调免疫反应中起着关键作用。在疟疾感染的红细胞阶段，T细胞增殖反应受到抑制，许多研究表明APC是造成这种现象的原因。在目前的研究中，我们研究了APC的T细胞活化功能的各个组成部分：共刺激和主要组织相容性复合体（MHC）11类蛋白的表达，处理和呈递抗原给T细胞的能力，以及支持细胞因子产生的能力。我们发现，在约氏疟原虫红细胞期感染的急性期，APC上调11类MHC和CD 80的表达，维持CD 86的表达，加工和呈递抗原，并支持γ干扰素的产生。然而，CD 11b（+）亚群产生一种或多种可溶性因子，其特异性抑制应答性CD 4 T细胞产生白细胞介素-2（IL-2）。该因子与前列腺素E-2 NO或转化生长因子β不同。数据表明，在疟疾感染期间观察到的IL-2抑制不是由于APC的功能缺陷，而是由主动抑制T细胞产生IL-2的因子的产生触发的。
Antigen-presenting cells (APC) play a key role in orchestrating immune responses. T-cell proliferative responses are inhibited during the erythrocyte stages of malaria infection, and a number of studies have suggested that APC are responsible for this phenomenon. In the present studies we examine individual components of the T-cell-activating function of APC: expression of costimulatory and major histocompatibility complex (MHC) class 11 proteins, the ability to process and present antigen to T cells, and the ability to support cytokine production. We find that during the acute phases of Plasmodium yoelii erythrocyte stage infection, APC upregulate the expression of class 11 MHC and CD80, maintain expression of CD86, process and present antigen, and support gamma interferon production. However the CD11b(+) subpopulation produces a soluble factor or factors that specifically inhibit interleukin-2 (IL-2) production by responding CD4 T cells. This factor is distinct from prostaglandin E-2 NO, or transforming growth factor beta. The data suggest that IL-2 suppression observed during malaria infection is not due to functional defects of APC but is triggered by production of a factor(s) that actively suppresses production of IL-2 by T cells.