Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic
Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic
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DOI：
10.13080/z-a.2018.105.047
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发表时间：
2018-01-01
影响因子：
0.9
通讯作者：
Karaman, Maja
中科院分区：
文献类型：
--
作者：
Bjelic, Dragana;Ignjatov, Maja;Karaman, Maja
丁香枯萎病。是大蒜的一种重要病害。发生在世界许多地区。然而，缺乏关于这些病原体生物防治的数据。芽孢杆菌因其在真菌病害生物防治中的潜在应用而备受关注。本研究的目的是从一系列土著芽孢杆菌中筛选出有效的生防菌株。与土壤隔离。筛选水解酶生产阳性的细菌分离株对镰刀菌属的抗真菌活性。从受感染的蒜瓣中分离出来的采用聚合酶链反应（PCR）技术对分离的细菌（16 SrDNA基因）和真菌（EF-1 α基因）进行分子鉴定，并检测芽孢杆菌属中抗菌脂肽（表面活性素、伊枯草菌素、芽孢杆菌霉素D和丰霉素）的生物合成基因。oxysporum F. sp. cepalidae、F. proliferatum、F. acuminatum和F.轮枝菌属（verticillioides）是蒜瓣腐烂病的病原菌。鉴定为枯草芽孢杆菌的四种细菌分离物在体外抗真菌活性测试期间表现出最高的拮抗作用（真菌生长减少高达71%），并引起大蒜腐烂感染的显着抑制（腐烂症状减少高达58%）。分析的抗真菌化合物参与的拮抗活性的检查菌株显示其产生抗生素脂肽表面活性素的能力。对B.枯草芽孢杆菌可作为大蒜腐烂病的潜在生防菌株。
Clove rot caused by Fusarium spp. is a very important disease of common garlic (Allium sativum L.) occuring in many areas of the world. However, there is a lack of data about biocontrol of these pathogens. Bacillus species are attractive for research due to their potential use in the biological control of fungal diseases. The aim of this study was to select effective biocontrol agents from a series of indigenous Bacillus spp. isolated from soil. Bacterial isolates positive for hydrolytic enzymes production were screened for antifungal activity against Fusarium spp. isolated from infected garlic cloves. Polymerase chain reaction (PCR) analyses were used for molecular identification of bacterial (16S rDNA gene) and fungal (EF-1 alpha gene) isolates, and detection of biosynthetic genes for antimicrobial lipopeptides (surfactin, iturin, bacillomycin D and fengycin) in Bacillus spp.The obtained results confirmed the presence of Fusarium tricinctum, F. oxysporum f. sp. cepae, F. proliferatum, F. acuminatum and F. verticillioides as the causal agents of garlic clove rot. Four bacterial isolates identified as Bacillus subtilis exhibited the highest antagonistic effect during in vitro testing of antifungal activity (up to 71% reduction in fungal growth), and caused a significant suppression of garlic clove infection (up to 58% reduction in rot symptoms) in situ. Analysis of the antifungal compounds involved in the antagonistic activity of the examined isolates revealed their ability to produce the antibiotic lipopeptide surfactin. The most effective isolates of B. subtilis could be used as potential biocontrol agents of garlic clove rot.