Reactive oxygen species participate in peroxynitrite-induced apoptosis in HL-60 cells
Reactive oxygen species participate in peroxynitrite-induced apoptosis in HL-60 cells
复制标题
DOI：
10.1006/bbrc.1996.5897
复制
发表时间：
1997-01-03
影响因子：
3.1
通讯作者：
Wong, PYK
中科院分区：
文献类型：
--
作者：
Lin, KT;Xue, JY;Wong, PYK
过氧亚硝酸盐（ONOO-）是一种由超氧化物（O-2）和一氧化氮（(NO)- n -）相互作用产生的生理产物，我们之前已经证实过氧亚硝酸盐诱导HL-60细胞凋亡，在本研究中，我们证实过氧亚硝酸盐在HL-60细胞中产生活性氧（ROS），在HL-60细胞中短暂暴露于ONOO-诱导荧光素升高，表明超氧阴离子的产生。外源性超氧化物歧化酶（SOD）是O-2（O-2）的清除剂。-)，完全废除了化学发光反应，进一步支持了这一观点。0 2(。过氧化氢酶（过氧化氢酶）的加入加剧了过氧化氢酶（过氧化氢酶）诱导的DNA片段化，而过氧化氢酶的加入则减弱了过氧化氢酶诱导的DNA片段化，这表明过氧化氢酶的产生有助于细胞凋亡过程。此外，用活性氧清除剂n -乙酰- l-半胱氨酸（15 mM）预处理HL-60细胞，完全清除了过氧化亚硝酸盐诱导的活性氧生成，有效抑制了ONOO诱导的细胞凋亡，进一步证实了这一假设。我们的研究结果表明，ONOO刺激的ROS形成可能是过氧亚硝酸盐介导的凋亡细胞死亡在完整细胞系统中增殖的机制。(C) 1997学术出版社
Peroxynitrite (ONOO-) is a physiological product generated by the interaction of superoxide (O-2(.-)) and nitric oxide ((NO)-N-.), We have previously shown that peroxynitrite induces apoptosis in HL-60 cells, In the present study, we demonstrated that peroxynitrite generates reactive oxygen species (ROS) in HL-60 cells, Brief exposure of HL-60 cells to ONOO- induced elevation of lucigenin chemiluminescence, indicating generation of superoxide anion. Exogenous superoxide dismutase (SOD), a scavenger of O-2(.-), fully abolished the chemiluminescence response, further supporting this notion. Following O-2(.-) generation, the accumulation of hydrogen peroxide (H2O2) was observed, The addition of SOD exacerbated but that of catalase attenuated peroxynitrite-induced DNA fragmentation, suggesting that this H2O2 production contributes to the apoptotic process, In addition, pre-treatment of HL-60 cells with N-acetyl-L-cysteine (15 mM), a ROS scavenger, fully scavenged peroxynitrite-elicited ROS generation and effectively inhibited ONOO--induced apoptosis, further enforcing this hypothesis, In summary, our results suggest that ONOO--stimulated ROS formation may serve as a mechanism for the propagation of peroxynitrite-mediated apoptotic cell death in an intact cell system. (C) 1997 Academic Press