A novel antisense long non-coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum
A novel antisense long non-coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum
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新型反义长非编码RNA通过调节禾谷镰刀菌GzmetE的表达参与无性和有性生殖
DOI：
10.1111/1462-2920.15399
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发表时间：
2021
影响因子：
5.1
通讯作者：
Jiasen Cheng
中科院分区：
文献类型：
--
作者：
Jie Wang;Wenping Zeng;Jiatao Xie;Yanping Fu;Daohong Jiang;Yang Lin;Weidong Chen;Jiasen Cheng
小麦赤霉病是一种世界性的重要病原菌，可引起小麦、大麦、玉米和其他谷物的赤霉病。lncrna在许多生物过程中发挥重要作用，但对其在丝状真菌中的功能和机制知之甚少。在这里，我们报道了一种天然的反义RNA，GzmetE‐AS，是从GzmetE的相反链转录而来的。gzmeteenen编码一种同型丝氨酸o‐乙酰转移酶，该酶在性发育和植物侵染中起重要作用。gzmete‐as的表达在分生阶段显著增加，而egzmete在有性生殖后期表达上调。过表达gzmete‐a抑制gzmete的转录。相比之下，gzmete的表达显著增加，inGzmetE‐转录终止株inGzmetE‐AS‐T。此外，GzmetE‐AS‐T产生更多的荚膜，促进子囊孢子排出，与GzmetE过表达菌株的表型相似。然而，过表达gzmete‐ASin∆dcl1/2菌株不能抑制gzmete的表达，并且在∆dcl1/2突变体中gzmetenat‐siRNA也显著减少。综上所述，我们发现了一种新的反义lncRNAGzmetE‐AS，它通过RNAi途径调节其反义基因egzmete参与无性生殖和有性生殖。我们的研究结果表明lncRNA在ofF.graminearum的发育中起着关键作用。
Fusarium graminearumis an important worldwide pathogen that causesFusariumhead blight in wheat, barley, maize and other grains. LncRNAs play important roles in many biological processes, but little is known about their functions and mechanisms in filamentous fungi. Here, we report that a natural antisense RNA,GzmetE‐AS, is transcribed from the opposite strand ofGzmetE.GzmetEencodes a homoserineO‐acetyltransferase, which is important for sexual development and plant infection. The expression ofGzmetE‐ASwas increased significantly during the conidiation stage, whileGzmetEwas upregulated in the late stage of sexual reproduction. Overexpression ofGzmetE‐ASinhibited the transcription ofGzmetE. In contrast, the expression ofGzmetEwas significantly increased inGzmetE‐AStranscription termination strainGzmetE‐AS‐T. Furthermore,GzmetE‐AS‐T produced more perithecia and facilitated the ascospore discharge, resembling the phenotype ofGzmetEoverexpressing strains. However, overexpression ofGzmetE‐ASin∆dcl1/2strain cannot inhibit the expression ofGzmetE, and theGzmetEnat‐siRNA is also significantly reduced in∆dcl1/2mutant. Taken together, we have identified a novel antisense lncRNAGzmetE‐AS, which is involved in asexual and sexual reproduction by regulating its antisense geneGzmetEthrough RNAi pathway. Our findings reveal that the lncRNA plays critical roles in the development ofF.graminearum.