Immunoglobulin genes of the kappa light chain type from two human lymphoid cell lines are closely related.
Immunoglobulin genes of the kappa light chain type from two human lymphoid cell lines are closely related.
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来自两种人淋巴细胞系的κ轻链型免疫球蛋白基因密切相关。
DOI：
10.1093/nar/12.18.6995
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发表时间：
1984
影响因子：
14.9
通讯作者：
H. Zachau
中科院分区：
文献类型：
--
作者：
H. Klobeck;G. Combriato;H. Zachau
作为人类 K 基因功能重排研究的第一步，我们使用小鼠 JK 克隆作为杂交探针，从胎盘 DNA 中克隆了种系 JK-CK 区域。然后使用人 JK-CK 区域的亚克隆来表征和克隆淋巴细胞系 Walker 和 Daudi 的重排 K 基因。 Walker 细胞系包含一种重排的和一种种系 K 等位基因（K+、KO；参考文献 1）。 Daudi 细胞（K+）中仅发现一个 K 基因。限制性图谱和DNA测序表明，两种细胞系的重排K基因密切相关。这些特征使得这两种细胞系特别适合研究K轻链基因的染色质结构。两个基因的 5' 侧翼 (388 bp) 相同，但 VK 基因片段本身之间存在 12% 的差异。这种情况可能反映了体细胞突变过程和/或基因转换等事件。
As a first step in our studies of functionally rearranged K genes of man we cloned the germline JK-CK region from placenta DNA employing a mouse JK clone as hybridization probe. Subclones of the human JK-CK region were then used to characterize and clone the rearranged K genes of the lymphoid cell lines Walker and Daudi. The Walker cell line contains one rearranged and one germline K allele (K+,KO; ref. 1). Only one K gene was found in Daudi cells (K+). Restriction mapping and DNA sequencing showed, that the rearranged K genes from both cell lines are closely related. These features make the two cell lines particularly suitable for studies on the chromatin structure of K light chain genes. The 5' flanks of the two genes (388 bp) are identical while there is a 12% divergence between the VK gene segments themselves. This situation may reflect somatic mutation processes and/or gene conversion like events.