Root pruning is effective in alleviating the inhibition of soybean growth caused by anaerobic stress for a short period
Root pruning is effective in alleviating the inhibition of soybean growth caused by anaerobic stress for a short period
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断根可有效缓解短期厌氧胁迫对大豆生长的抑制
DOI：
10.1016/j.jia.2022.08.028
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发表时间：
2023
影响因子：
4.8
通讯作者：
Iijima Morio
中科院分区：
文献类型：
--
作者：
Yamane Koji;Mariyama Miki;Hirooka Yoshihiro;Iijima Morio
大豆是重要的旱地作物，但其生产力往往受到涝害造成的厌氧胁迫的限制。在环境限制下调节根系生长的能力是适应不断变化的环境的重要生理特性，而根系修剪是一种再生根系的人工技术。在本研究中，我们研究了大豆的根修剪是否可以有效减轻厌氧胁迫的抑制作用。大豆植株在发芽、营养阶段1（V1）和繁殖阶段1（R1）阶段受到厌氧胁迫，然后在胁迫处理后立即对植物进行根修剪处理。发芽阶段的大豆植株在缺氧（N2处理）后进行根冠和尖端去除处理。去除根冠比去除根尖（距根尖5毫米）更能有效抑制缺氧的抑制作用。缺氧和不去除根冠的大豆植株地上部干重分别是对照的51.2和73.8%，而缺氧和不去除根冠的大豆植株的根干重分别是对照的43.2和62.8%。由于根冠去除有效地促进了厌氧胁迫后大豆的生长，因此根冠可能是胁迫记忆机制的候选组织。当大豆生长V1期植株受到厌氧胁迫时，R1期厌氧胁迫处理的大豆植株的分枝数、主茎荚重、根长和根表面积均较对照显着下降。相比之下，胁迫处理后立即修剪根部（距尖端 2 毫米）可增强根部生长、分枝数量和豆荚重量。剪根处理的植株分枝数、荚重、根长和根表面积分别是厌氧胁迫处理的1.13、1.14、1.12和1.13倍。在厌氧条件下，大豆根的根分生组织、小柱和皮层细胞中观察到质壁分解。然而，在经过厌氧胁迫处理的植物中，剪根后新出现的根没有受到损害。这些结果表明，根部修剪可有效促进厌氧胁迫后大豆的生长。这种效果可能是由于恢复期间健康侧根的再生和伸长。当大豆植株在R1期受到厌氧胁迫时，胁迫处理后立即剪根是无效的。因此，仅使用根修剪来抑制由于繁殖阶段的厌氧应激而导致的生长减少可能是困难的。
Soybean is an important upland crop, but its productivity is often limited by anaerobic stress caused by waterlogging. The ability to adjust root growth under environmental constraints is an important physiological trait for adapting to an ever-changing environment, and root pruning is an artificial technique for regenerating the root system. In the present study, we investigated whether root pruning in soybean can effectively alleviate the inhibitory effects of anaerobic stress. Soybean plants were affected by anaerobic stress at the germination, vegetative stage 1 (V1), and reproductive stage 1 (R1) stages, and then the plants were treated with root pruning just after the stress treatment. Soybean plants at the germination stage were treated with root cap and tip removals after hypoxia (N2treatment). Root cap removal was more effective in suppressing the inhibitory effects of hypoxia than root tip removal (5 mm from the tip). The shoot dry weights of the soybean plants with and without root cap removal after hypoxia were 51.2 and 73.8% of the control, respectively, while the root dry weights of plants with and without root cap removal after hypoxia were 43.2 and 62.8% of the control, respectively. As root cap removal effectively enhanced soybean growth after anaerobic stress, the root cap may be the candidate tissue for the stress memory mechanism. When soybean plants at the V1 stage of growth were affected by anaerobic stress, the branch number, pod weight in the main stem, root length, and root surface area of the soybean plants treated with anaerobic stress at the R1 stage significantly decreased compared with those of the control. In contrast, root pruning (2 mm from the tip) immediately after the stress treatment enhanced root growth, branch number, and pod weight. The branch number, pod weight, root length, and root surface area of the plants treated with root pruning were 1.13, 1.14, 1.12, and 1.13 times higher than those of plants treated with anaerobic stress. Plasmolysis was observed in the root meristem, columella, and cortical cells in soybean roots subjected to anaerobic conditions. However, damage was not observed in the newly emerged roots after root pruning in plants treated with anaerobic stress. These results suggested that root pruning is effective in enhancing soybean growth after anaerobic stress. This effectiveness may be due to the regeneration and elongation of healthy lateral roots during the recovery period. When soybean plants were affected by anaerobic stress at the R1 stage, root pruning just after the stress treatment was ineffective. Thus, suppressing the growth reduction due to anaerobic stress at reproductive stages using only root pruning may be difficult.
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DOI：
10.1007/s10725-008-9340-1
发表时间：
2009
期刊：
Plant growth regulation (Print)
影响因子：
--
作者：
Shou;Fengmin Li;Bingcheng Xu;Zhanbin Huang
通讯作者：
Zhanbin Huang
影响因子：
6.9
作者：
Iijima, M;Higuchi, T;Bengough, AG
通讯作者：
Bengough, AG
162份大豆材料苗期缺氧条件下根系发育表型变异
影响因子：
2.5
作者：
Keisuke Suematsu;T. Abiko;V. Nguyen;T. Mochizuki
通讯作者：
T. Mochizuki
影响因子：
2.5
作者：
M. Iijima;Y. Hirooka;Yoshimasa Kawato;Y. Watanabe;K. Wada;Nodoka Shinohara;Pamwenafye I. Nanhapo
通讯作者：
M. Iijima;Y. Hirooka;Yoshimasa Kawato;Y. Watanabe;K. Wada;Nodoka Shinohara;Pamwenafye I. Nanhapo
使用窄根通道仪将自然根嫁接应用于一年生作物
影响因子：
2.5
作者：
M. Iijima;K. Yamane;Y. Hirooka;K. Wada;Y. Okada;Miki Mariyama;Y. Watanabe
通讯作者：
Y. Watanabe