Optical Injection of Gold Nanoparticles into Living Cells
Optical Injection of Gold Nanoparticles into Living Cells
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DOI：
10.1021/nl504497m
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发表时间：
2015-01-01
期刊：
影响因子：
10.8
通讯作者：
Feldmann, Jochen
中科院分区：
文献类型：
--
作者：
Li, Miao;Lohmueller, Theobald;Feldmann, Jochen
利用光将纳米级物体可控地注射到活细胞中为开发新的分子递送策略或细胞内生物传感器应用提供了有希望的前景。在这里，我们展示了来自溶液的单个金纳米颗粒可以用连续波激光束在活细胞表面上形成图案。在第二步中，我们演示了如何通过等离子体激元加热和光学力的组合将相同的粒子注入细胞。我们发现，短的暴露时间足以使细胞膜破裂，并将颗粒注入细胞，存活率> 70%。
The controlled injection of nanoscopic objects into living cells with light offers promising prospects for the development of novel molecular delivery strategies or intracellular biosensor applications. Here, we show that single gold nanoparticles from solution can be patterned on the surface of living cells with a continuous wave laser beam. In a second step, we demonstrate how the same particles can then be injected into the cells through a combination of plasmonic heating and optical force. We find that short exposure times are sufficient to perforate the cell membrane and inject the particles into cells with a survival rate of >70%.