An interlaboratory comparison of methods used to assess antioxidant potentials
An interlaboratory comparison of methods used to assess antioxidant potentials
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DOI：
10.1111/j.1467-2494.2006.00311.x
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发表时间：
2006-04-01
影响因子：
2.3
通讯作者：
Wollenweber, U.
中科院分区：
文献类型：
--
作者：
Buenger, J.;Ackermann, H.;Wollenweber, U.
许多分析方法被用来测量物质的抗氧化活性，但对实验室之间测试结果的可比性知之甚少。在对一个实验室进行的广泛方法进行初步评价之后。选择12-二苯基-1-苦基肼（DPPH）测定、trolox当量抗氧化能力（TEAC）测定、脂质测定（或2.2 '-偶氮二（2-胺丙烷）（ABAP）测定）和硫代巴比妥酸（TBA）测定在实验室间研究中进行评价。trolox，生育酚的抗氧化潜力。脂色满-6抗坏血酸4-甲基-儿茶素和/或3，5-二叔丁基-4-羟基甲苯（BHT）。然后，这些方法进行了评价，在其重现性和分类性能。根据本研究的结果，DPPH测定法和TEAC测定法在一个实验室内和实验室之间的重现性和灵敏度方面均获得了最佳结果。然后将实验室间研究的结果与从市售光化学发光（PCL）试剂盒获得的单中心结果进行比较。为了评估化学数据到生物系统的可转移性，还将其与使用基于细胞的二氯二氢荧光素（DCFH）测定法获得的单中心结果进行了比较。
Many analytical methods are used to measure the antioxidative activity of substances yet little is known about the comparability of the test results between laboratories. After an initial evaluation of a broad range of methods conducted by one laboratory. the 12-diphenyl-1-picrylhydrazyl (DPPH) assay, the trolox equivalent antioxidant capacity (TEAC) assay, the lipid assay (or 2.2'-azobis (2-aminepropane) (ABAP) assay) and the thiobarbituric acid (TBA) assay were selected to be evaluated in the interlaboratory study. The antioxidative potentials of trolox, tocopherol. lipochroman-6, ascorbic acid. 4-methyl-brenzcatechin, and/or 3,5-di-tert-butyl-4-hydroxytoluene (BHT) were assessed using each of the methods. These methods were then evaluated in respect of their reproducibility and classification properties. Based on the results of this study, the DPPH assay followed by the TEAC assay yielded the best results based on reproducibility and sensitivity both within one laboratory and between laboratories. The results of the interlaboratory study were then compared with the single center results obtained from the commercially available photochemolumiescence (PCL) kit. To assess the transferability of chemical data to biological systems, they were also compared with the single center results obtained using the cell-based Dichlorodihydrofluoresceine (DCFH) assay.