Stress-Related Neuronal Clusters in Sublenticular Extended Amygdala of Basal Forebrain Show Individual Differences of Positions
Stress-Related Neuronal Clusters in Sublenticular Extended Amygdala of Basal Forebrain Show Individual Differences of Positions
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DOI：
10.3389/fncir.2020.00029
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发表时间：
2020-05-28
影响因子：
3.5
通讯作者：
Ichijo, Hiroyuki
中科院分区：
文献类型：
--
作者：
Kanemoto, Munenori;Nakamura, Tomoya;Ichijo, Hiroyuki
为了了解小鼠基底前脑中情绪的功能神经元回路，用即刻早期基因产物(Zif268/Egr1和c-Fos)的免疫组织化学方法研究了小鼠神经元的激活模式。在所有受检的小鼠中，在杏仁核(EA)和苍白球(GP)之间的两侧都发现了30-50个表达Zif268的神经元簇，通常被称为豆状核下扩展杏仁核(SLEA)。GAD65-mCherry小鼠的GABA能神经元中有79.9+/-3.0%。胆碱能标志物胆碱乙酰转移酶和GP标志物小白蛋白、前脑啡肽和FoxP2的表达表明，这些神经元不同于电针和GP中已知的神经元类型，因此，我们将其命名为豆状核下扩展杏仁核Zif268/Egr1表达神经元簇(Slea-zNC)。杏仁核豆状核下延伸的Zif268/Egr1表达的神经元团参与了应激处理，因为束缚应激(RS)后，Slea-zNC中观察到细胞数量增加，而地西潘抑制了这种诱导。Slea-zNC定位显示，Slea-zNC的位置和排列各不相同，Slea-zNC分布不对称，主要位于前连合(AC)和GP后端之间的中间区域。然而，RS激活后，Slea-zNCs的细胞总数在左右半球之间是相容的。因此，Slea-zNC呈不对称分布，但不呈偏侧分布。由于Zif268和c-Fos激活的时间进程不同，RSS的序贯双重处理使我们能够区分由第一和第二RS激活的Slea-zNC。结果支持相同的Slea-zNC对第一个和第二个RS作出反应，这也适用于所有的Slea-zNC。因此，我们得出结论，在RS下，簇的位置在每只小鼠中是不变的，但在不同的小鼠之间分布不同。我们将这些新发现的神经元簇命名为应激相关神经元簇，Slea-zNC，它们被认为是“激活岛”的新功能单位。此外，Slea-zNC在所有被检查的小鼠中都位于不同的位置，显示出它们的位置的个体差异。
To understand functional neuronal circuits for emotion in the basal forebrain, patterns of neuronal activation were examined in mice by immunohistochemistry of immediate-early gene products (Zif268/Egr1 and c-Fos). In all mice examined, clusters of 30-50 neurons expressing Zif268 were found on both sides in the area between the extended amygdala (EA) and globus pallidus (GP), generally designated as sublenticular extended amygdala (SLEA). The clusters consisted of 79.9 +/- 3.0% of GABAergic neurons in GAD65-mCherry mice. The expression of the cholinergic marker choline acetyltransferase and the GP markers parvalbumin, proenkephalin, and FoxP2 indicated that these neurons were different from known types of neurons in the EA and GP; therefore, we named them the sublenticular extended amygdalar Zif268/Egr1-expressing neuronal cluster (SLEA-zNC). Sublenticular extended amygdalar Zif268/Egr1-expressing neuronal clusters participated in stress processing because increasing numbers of cells were observed in SLEA-zNCs after exposure to restraint stress (RS), the induction of which was suppressed by diazepam treatment. Mapping SLEA-zNCs showed that their positions and arrangement varied individually; SLEA-zNCs were distributed asymmetrically and tended to be situated mainly in the middle region between the anterior commissure (AC) and posterior end of the GP. However, the total cell number in SLEA-zNCs was compatible between the right and left hemispheres after activation by RS. Therefore, SLEA-zNCs were distributed asymmetrically but were not lateralized. Because time courses of activation differed between the Zif268 and c-Fos, the sequential dual treatment of RSs enabled us to differentiate SLEA-zNCs activated by the first and second RS. The results supported that the same SLEA-zNCs responded to both the first and second RS, and this also applied for all SLEA-zNCs. Thus, we concluded that the cluster positions were invariable under RS in each mouse but were distributed differently between individual mice. We name these newly identified neuronal clusters as stress-related neuronal clusters, SLEA-zNCs, which are considered to be novel functional units of "islands of activation." Moreover, SLEA-zNCs were situated at different positions in all mice examined, showing individual differences in their positions.