Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system
Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system
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DOI：
10.1038/nplants.2015.145
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发表时间：
2015-09-28
期刊：
影响因子：
18
通讯作者：
Voytas, Daniel F.
中科院分区：
文献类型：
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作者：
Baltes, Nicholas J.;Hummel, Aaron W.;Voytas, Daniel F.
为了减少双子星病毒感染造成的作物损失，我们以大豆黄矮病毒（BeYDV）基因组为目标，利用CRISPR-Cas（聚集的、有规则间隔的短回文重复序列- crispr相关蛋白）系统进行破坏。利用基于beydv的复制子进行的瞬时分析显示，CRISPR-Cas试剂在病毒基因组中引入了突变，并减少了病毒拷贝数。表达CRISPR-Cas试剂的转基因植物在BeYDV刺激下减少了病毒载量和症状，从而展示了一种新的抗双病毒工程策略。
To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.